Therapeutic deep eutectic solvent-based microemulsion enhances anti-inflammatory efficacy of curcuminoids and aromatic-turmerone extracted from Curcuma longa L. Issue 40 (12th September 2022)

• Record Type: Journal Article
• Authors: Supaweera, Nassareen; Chulrik, Wanatsanan; Jansakun, Chutima; Bhoopong, Phuangthip; Yusakul, Gorawit; Chunglok, Warangkana
• Is Part Of: RSC advances. Volume 12:Issue 40(2022)
• Page Start: 25912
• Page End: 25922
• Publication Date: 2022-09-12
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra03656h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A novel HDES-based microemulsion system with anti-inflammatory activity serves as an extraction solvent of Curcuma longa L. Abstract : To diminish chemical waste and improve the delivery of Curcuma longa L. (CL) constituents, microemulsions based on hydrophobic deep eutectic solvents (HDESs) were designed as ready-to-use solvents for CL extraction. The microemulsion (ME) of the ME-23 formulation (HDES/Tween 80 : propylene glycol (1 : 1)/water, 25/70/5) displayed CL extraction yields of 1.69, 3.04, 7.36, and 1.39 wt% of bisdemethoxycurcumin, demethoxycurcumin, curcumin, and aromatic-turmerone, respectively. The ME-23 without CL chemical constituents and ME-23-based CL extract inhibited NO production with an IC50 value of 0.0136 ± 0.0023%v/v and a curcumin IC50 value of 75.2 ± 6.7 nM, respectively, and simultaneously lowered inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-6, and IL-1β production in lipopolysaccharide-activated murine macrophages. Authentic curcumin in ME-23 possessed superior NO inhibitory activity, which was 103-fold more effective than curcumin prepared in the conventional solvent dimethyl sulfoxide. ME-23 was also capable of delivering curcumin into murine macrophages. After 30 days of storage in HDES and HDES-based ME, curcumin remained more than 90%. ME-23 provides advantages for CL extraction, constituent delivery, and anti-inflammatory functions that can be applied to pharmaceutical and cosmetic products.