Multifunctional-high resolution imaging plate based on hydrophilic graphene for digital pathology. (10th December 2022)

• Record Type: Journal Article
• Authors: Lee, Geonhee; Oh, Yuna; Nam, Jung Tae; Ji, Seulgi; Jang, A-Rang; Jeong, Du Won; Kang, MinSoung; Lee, Sun Sook; Chae, Soosang; Cho, Donghwi; Hwang, Jun Yeon; Lee, Kyungeun; Lee, Jeong-O
• Is Part Of: Nanotechnology. Volume 33:Number 50(2022)
• Publication Date: 2022-12-10
• Subjects: graphene -- polydimethylsiloxane (PDMS) -- scanning electron microscope (SEM) -- correlative light and electron microscopy (CLEM); Nanotechnology -- Periodicals; Nanotechnology -- Periodicals; Nanotechnology; Publications périodiques; Nanotechnologies; Periodicals; 620.5
• DOI: 10.1088/1361-6528/ac9143
• Languages: English
• ISSNs: 0957-4484

Abstract

Abstract: In the present study, we showed that hydrophilic graphene can serve as an ideal imaging plate for biological specimens. Graphene being a single-atom-thick semi-metal with low secondary electron emission, array tomography analysis of serial sections of biological specimens on a graphene substrate showed excellent image quality with improved z -axis resolution, without including any conductive surface coatings. However, the hydrophobic nature of graphene makes the placement of biological specimens difficult; graphene functionalized with polydimethylsiloxane oligomer was fabricated using a simple soft lithography technique and then processed with oxygen plasma to provide hydrophilic graphene with minimal damage to graphene. High-quality scanning electron microscopy images of biological specimens free from charging effects or distortion were obtained, and the optical transparency of graphene enabled fluorescence imaging of the specimen; high-resolution correlated electron and light microscopy analysis of the specimen became possible with the hydrophilic graphene plate.