Anti-inflammation effects of highly purified low-Mw RG-I pectins on LPS-activated macrophages. (November 2021)

• Record Type: Journal Article
• Authors: Wu, Dongmei; Zheng, Xiaoliang; Hu, Weiwei; Zhu, Kai; Yu, Chengxiao; He, Qiaojun; Linhardt, Robert J.; Ye, Xingqian; Chen, Shiguo
• Is Part Of: Bioactive carbohydrates and dietary fibre. Number 26(2021)
• Publication Date: 2021-11
• Subjects: RG-I pectin -- Arabinan -- Sidechains -- Anti-inflammatory -- Molecular weight -- Pro-inflammatory cytokines -- IL-6; Functional foods -- Periodicals; Fiber in human nutrition -- Periodicals; Carbohydrates -- Periodicals; Glycoproteins -- Periodicals; Functional Food -- Periodicals; Dietary Fiber -- Periodicals; Carbohydrates -- Periodicals; Carbohydrates; Fiber in human nutrition; Functional foods; Glycoproteins; Periodicals; 613.28
• DOI: 10.1016/j.bcdf.2021.100283
• Languages: English
• ISSNs: 2212-6198

Abstract

Abstract: Pectic oligosaccharides are viewed as excellent immunomodulator, but the specific molecular weight (Mw ) range required for anti-inflammatory effects remains unclear. In this study, citrus pectins was depolymerized by the ultrasound/H2O2/Vc system and the pectic mixtures was separated through the superdex 30 chromatography column. Three highly purified pectin fractions (USP1, USP2, USP3) were obtained, with the Mw of 2713 g/mol, 3683 g/mol, and 7469 g/mol. All of them were RG-I pectins and USP3 contained more arabinan sidechains than USP1 and USP2. In vitro anti-inflammation assays based on LPS-induced RAW264.7 macrophages indicated that these pectins could suppress the NO release, ROS production and down-regulated the level of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in a Mw -dependent manner. The inhibitory effects increased in the order of USP1<USP2<USP3. Meanwhile, the regulation of pro-inflammatory cytokines were closely related to the content of arabinan sidechains in pectin. Thus, low-Mw pectins with Mw >3KD should be effective as a prophylactic agent against inflammatory diseases in functional food. Highlights: Three highly purified low-Mw pectins (USP1, USP2, USP3) are prepared. These USP fractions significantly reduced the NO and ROS production. The level of inflammatory cytokines IL-1β, IL-6 and TNF-α were down-regulated. The arabinan sidechains were the key structural domain for suppressing IL-6 secretion. The anti-inflammatory effects were enhanced with the increasing of Mw (7KD>3KD>2KD).