Amelioration of hydrolyzed guar gum on high-fat diet-induced obesity: Integrated hepatic transcriptome and metabolome. (1st December 2022)

• Record Type: Journal Article
• Authors: Fu, Xiaodan; Liu, Zhemin; Li, Rong; Yin, Junyi; Sun, Han; Zhu, Changliang; Kong, Qing; Mou, Haijin; Nie, Shaoping
• Is Part Of: Carbohydrate polymers. Volume 297(2022)
• Publication Date: 2022-12-01
• Subjects: ACC acetyl-CoA carboxylase; -- Acsl1 long-chain acyl-CoA synthases -- AGPAT acylglycerol-phosphate acyltransferase -- ALT alanine aminotransferase -- AST aspartate aminotransferase -- AUC area under the curve -- BH Benjamini and Hochberg -- Cd36 cluster of differentiation 36 -- Chka choline kinase α -- ChREBP carbohydrate-responsive element-binding protein -- Cpt1a carnitine palmitoyltransferase 1α -- DEGs differentially expressed genes -- Dgat2 diacylglycerol O-acyltransferase 2 -- DNL de novo lipogenesis -- ESI electrospray ionization -- FA fatty acid -- Fabp4 fatty acids binding protein 4 -- Fasn fatty acid synthase -- FC fold change -- GMLP low molecular weight hydrolyzed guar gum -- GPAT glycerol-phosphate acyltransferase -- HDLC high-density lipoprotein cholesterol -- HFD high-fat diet -- HGH HFD supplemented with high dose GMLP -- HGL HFD supplemented with low dose GMLP -- HO HFD supplemented with orlistat -- HOMA-IR homeostasis model assessment of insulin resistance -- H&E hematoxylin and eosin -- GSH-Px glutathione peroxidase -- LDLC low-density lipoprotein cholesterol -- LXR-RXR liver X receptor-retinoid X receptor -- MWCO molecular weight cut-off -- Mw molecular weight -- G/M molar ratio of galactose to mannose -- MDA malondialdehyde -- NAFLD nonalcoholic fatty liver disease -- NEFA non-esterified fatty acid -- NCD normal control diet -- NDCs non-digestible carbohydrates -- NGH NCD supplemented with high dose GMLP -- QC quality control -- OGTT oral glucose tolerance test -- OPLS-DA orthogonal partial least squares discriminant analysis -- PAP phosphatidic acid phosphorylase -- PCA principal component analysis -- Pparα peroxisome proliferator-activated receptor α -- ROS reactive oxygen species -- SCFAs short-chain fatty acids -- SD standard deviation -- RT-PCR real-time quantitative polymerase chain reaction -- SOD superoxide dismutase -- Srebp1c sterol response element binding protein 1c -- TC total cholesterol -- TG triglycerides -- TPM transcripts per million reads -- VIP variable importance; Low molecular weight hydrolyzed guar gum -- Obesity -- Hyperlipidemia -- Hepatic transcriptome -- Hepatic metabolome -- Fatty acid metabolism; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120051
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Hydrolyzed guar gum has gained attention as an anti-obesity agent; however, few studies have focused on its role in amelioration of hepatic-associated metabolic processes. Here, the anti-obesity effect of low molecular weight hydrolyzed guar gum (GMLP, 1–10 kDa) on high-fat diet (HFD)-fed C57BL/6 J mice was investigated via transcriptome and metabolome in liver. GMLP reduced body weight gain and hepatic lipid accumulation dose-dependently, regulated blood lipid levels, and improved liver damage in HFD-fed mice. Integrated transcriptome and metabolome indicated that GMLP mainly altered lipid metabolism pathways (glycerophospholipid metabolism, glycerolipid metabolism, and fatty acid degradation), reduced disease biomarkers of ethyl glucuronide and neopterin, and increased levels of choline, flavin adenine dinucleotide, and pantetheine metabolites. Real-time quantitative PCR showed that GMLP downregulated key genes involved in de novo lipogenesis and triacylglycerol synthesis, while promoting fatty acid oxidation and choline synthesis. This study provides a theoretical basis for GMLP treatment in future clinical applications. Graphical abstract: Unlabelled Image