Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism. (1st December 2022)

• Record Type: Journal Article
• Authors: Li, Lu; Xu, Weijie; Luo, Yinzhu; Lao, Chunqin; Tong, Xueli; Du, Junxi; Huang, Bihong; Li, Di; Chen, Jingmian; Ye, Huiling; Cong, Feng; Guo, Xiaofeng; Li, Jiejing
• Is Part Of: Carbohydrate polymers. Volume 297(2022)
• Publication Date: 2022-12-01
• Subjects: ABPA1 Aloe barbadensis polymeric acemannan 1 -- AVBEC Aloe vera barbadensis extract C -- ATP adenosine triphosphate -- COVID-19 coronavirus disease 2019 -- DAPI diamidinyl phenyl indole -- ETC Electron Transport Chain -- FITC fluorescein isothiocyanate -- GC–MS gas chromatography-mass spectrometer -- H&E haematoxylin and eosin -- HPGPC high-performance gel permeation chromatography -- HRP horseradish peroxidase -- IAV Influenza A Virus -- IHC immunohistochemistry -- LD50 lethal dose -- LPS lipopolysaccharide -- NMR nuclear magnetic resonance -- OD optical density -- PBS phosphate buffer saline -- OXPHOS oxidative phosphorylation -- PFUs plaque forming units -- PVDF polyvinylidene fluoride -- ROS reactive oxygen species -- SARS-CoV-2 severe acute respiratory syndrome coronavirus 2 -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis -- TCID50 50 % tissue culture infective dose -- TMRE tetramethylrhodamine ethyl ester; Cytokine storm -- Acute pneumonia -- Aloe polysaccharide -- Macrophage; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120032
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The cytokine storm is highly associated with inflammatory-type disease severity and patients' survival. Plant polysaccharides, the main natural phytomedicine source, have a great potential to be an effective drug to treat cytokine storm. Herein we found that a polymeric acemannan (ABPA1) isolated from Aloe Vera Barbadensis extract C (AVBEC) exerted prominent inhibitory effects on inflammation-induced cytokine storm. The results displayed that ABPA1 effectively suppressed LPS-induced proinflammatory cytokines release in vitro . Moreover, ABPA1 treatment alleviated the cytokine storm and tissue damage in LPS- and IAV-induced mouse pneumonia models, and altered the phenotypic balance of macrophages in lung tissues. Functionally, ABPA1 enhanced macrophage M2 polarization and phagocytosis in RAW264.7 cells and inhibited LPS-induced M1 polarization. Mechanistically, ABPA1 enhanced mitochondrial metabolism and OXPHOS through activated PI3K/Akt/GSK-3β signalling pathway. Overall, our findings suggest that ABPA1 may modulate macrophage activation and mitochondrial metabolism by targeting PI3K/Akt/GSK-3β signalling pathway, thereby alleviating cytokine storm and inflammation. Graphical abstract: Unlabelled Image