Hemoglobin-binding α-synuclein levels in erythrocytes are elevated in patients with multiple system atrophy. (15th October 2022)

• Record Type: Journal Article
• Authors: Zhang, Xinning; Wang, Sushi; Li, Xuran; Li, Xin; Ran, Weizheng; Liu, Chengwei; Tian, Wenyang; Yu, Xiaohan; Wu, Chunlei; Li, Pengjie; Li, Nannan; Wei, Yifo; Wang, Yao; Yu, Shun; Chen, Zhigang
• Is Part Of: Neuroscience letters. Volume 789(2022)
• Publication Date: 2022-10-15
• Subjects: PD Parkinson's disease -- DLB dementia with Lewy bodies -- MSA multiple system atrophy -- α-syn α-synuclein -- LB Lewy body -- GCI glial cytoplasmic inclusion -- NCI neuronal cytoplasmic inclusion -- NNI neuronal nuclear inclusion -- t-α-syn total α-synuclein -- o-α-syn oligomeric α-synuclein -- p-α-syn phosphorylatedα-synuclein -- exo-α-syn exosome-derive α-synuclein -- CSF cerebrospinal fluid -- ERC Erythrocyte -- Hb hemoglobin -- Hb-α-syn hemoglobin-binding α-syn -- HC healthy controls -- MSA-P MSA with predominant parkinsonism -- MSA-C MSA with predominant cerebellar ataxia -- ELISA enzyme-linked immunosorbent assay -- UMSARS Unified Multiple System Atrophy Rating Scale -- ROC receiver operating characteristic curve -- AUC area under the curve; Multiple system atrophy -- α-Synuclein -- Hemoglobin; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2022.136868
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Interaction between Hb and α-syn is present in human peripheral erythrocytes. Hb-α-syn levels in erythrocytes increased in patients with MSA. Erythrocytic Hb-α-syn could reflect α-syn accumulation in MSA patients. Abstract: Previous studies have shown that α-synuclein (α-syn) accumulation in the normal aging brain is associated with a parallel increase in hemoglobin-binding α-syn (Hb-α-syn) in the brain and peripheral erythrocytes (ERCs), indicating that Hb-α-syn levels in ERCs may reflect the α-syn changes in the brain. However, if there is any change in ERC Hb-α-syn levels in disease condition is unclear. In this study, Hb-α-syn levels in ERCs from 149 Patients with multiple system atrophy (MSA) and 149 healthy controls (HCs) were measured by enzyme linked immunosorbent assay (ELISA). The results showed that Hb-α-syn levels in ERCs were significantly increased in MSA patients in comparison with those in HCs (777.84 ± 240.82 ng/mg vs 508.84 ± 162.57 ng/mg, P < 0.001). Receiver operating characteristic curve (ROC) indicated that increased Hb-α-syn in ERCs could discriminate MSA patients from HCs, with a sensitivity of 71.8%, a specificity of 80.5%, and an area under the curve (AUC) of 0.837. The positive and negative predictive values at a cut-off value of 616.12 ng/mg were 78.7% and 74.1%, respectively. However, the increase in Hb-α-syn levels did not show any association with the age of onset and consultation, disease duration, and UMSARS (I-IV) score. This pilot study suggests that ERC Hb-α-syn is increased in MSA patients and could evaluate α-syn accumulation in the brain of patients.