Rapid Determination of Cephalexin in Animal-Derived Food by an Indirect Competitive Enzyme-Linked Immunosorbent Assay. (13th September 2022)

• Record Type: Journal Article
• Authors: Wu, Shuangmin; Shen, Wei; Chen, Yushuang; Zhu, Yongli; Hou, Ren; Dai, Menghong; Peng, Dapeng
• Is Part Of: Analytical letters. Volume 55:Number 17(2022)
• Page Start: 2803
• Page End: 2816
• Publication Date: 2022-09-13
• Subjects: Animal-derived food -- cephalexin -- high-performance liquid chromatography-mass spectrometry -- indirect competitive enzyme-linked immunosorbent assay -- monoclonal antibody; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2022.2072857
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: Cephalexin (CFE) residues in animal-derived food and its deleterious effects on human health has attracted extensive attention. In this study, a specific anti-cephalexin monoclonal antibody (mAb) was used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with a half-maximal inhibitory concentration (IC50 ) of 1.83 µg/L that was suitable for the determination of cephalexin in animal-derived foods. The limit of detection (LOD) and limit of quantitation (LOQ) for cephalexin were from 0.44 to 0.58 µg/kg and 0.57 to 0.67 µg/kg, respectively. The recovery was from 90.1 to 103.6% with coefficients of variation (CV) less than 10.0%. In addition, the established ELISA method and high-performance liquid chromatography – mass spectrometry (HPLC-MS/MS) were used to analyze the samples with good correlation between the results. The results show that the established ic-ELISA is suitable for the determination of cephalexin in animal-derived food.