Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure. Issue 7 (21st September 2021)

• Record Type: Journal Article
• Authors: López‐Rodríguez, Juan Carlos; Rodríguez‐Coira, Juan; Benedé, Sara; Barbas, Coral; Barber, Domingo; Villalba, María Teresa; Escribese, María Marta; Villaseñor, Alma; Batanero, Eva
• Is Part Of: Clinical and translational allergy. Volume 11:Issue 7(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-21
• Subjects: air‐liquid interface culture -- bronchial epithelium -- Der p 1 -- metabolome -- respiratory allergy; Allergy -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Hypersensitivity -- Periodicals; Immune System Phenomena -- Periodicals; 616.97005
• DOI: 10.1002/clt2.12051
• Languages: English
• ISSNs: 2045-7022

Abstract

Abstract: Background: Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine‐protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development. Methods: Calu‐3 cells were cultivated in air‐liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were performed using liquid chromatography and capillary electrophoresis both coupled to mass spectrometry. Results: We showed that epithelial barrier disruption by Der p 1 was associated with a specific metabolic profile, which was highly dependent on the state of the epithelium at the time of contact. Moreover, an apical‐basolateral distribution of the metabolites was also observed, indicating a compartmentalization of the response with differential metabolic patterns. A number of metabolites were changed by Der p 1, mainly related to amino acids metabolism, such as L‐arginine, L‐kynurenine and L‐methionine. Conclusion: This work is the first report on the metabolic response in human bronchial epithelial cells associated with cysteine‐protease Der p 1 activity, which could contribute to allergy development. Moreover, it supports a reformulated epithelial barrier hypothesis that might help to explain allergies and their increasing prevalence.