Facile preparation of Fe3O4@Pt nanoparticles as peroxidase mimics for sensitive glucose detection by a paper-based colorimetric assay. Issue 9 (28th September 2022)

• Record Type: Journal Article
• Authors: He, Ye; Wang, Panlin; Chen, Xiaojing; Li, Yahuang; Wei, Jiajun; Cai, Guoxi; Aoyagi, Kiyoshi; Wang, Wenxiang
• Is Part Of: Royal Society open science. Volume 9:Issue 9(2022)
• Publication Date: 2022-09-28
• Subjects: Fe3O4@Pt NPs -- nanoenzyme -- glucose -- colorimetric -- paper-based device; Science -- Periodicals; 500
• DOI: 10.1098/rsos.220484
• Languages: English
• ISSNs: 2054-5703

Abstract

Abstract : A simple strategy to rapidly detect glucose was developed by utilizing core (Fe3 O4 )–shell (Pt) magnetic nanoparticles (Fe3 O4 @Pt NPs) as a nanoenzyme and a paper-based colorimetric sensor. In the presence of H2 O2, Fe3 O4 @Pt NPs catalyze the redox reaction of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) and generate a colour change from colourless to blue. On this basis, a colorimetric glucose sensing method assisted by glucose oxidase (GOx) was developed. Under the optimal conditions, the detection limits of the proposed assay for H2 O2 and glucose were 0.36 µM and 1.27 µM, respectively. Furthermore, the fabricated colorimetric method was successfully applied to analyze glucose concentrations by using a paper device as a measuring platform without a spectrometer. In addition, this method exhibited satisfactory recovery for glucose detection in human serum samples and urine samples, which satisfied the requirements for normal detection of real samples. This study provides a good candidate for health monitoring of glucose and also expands the applications of nanoenzymes and paper-based colorimetric assays in point-of-care testing.