Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis. (23rd August 2022)

• Record Type: Journal Article
• Authors: Young, Bernadette C; Bush, Stephen J; Lipworth, Sam; George, Sophie; Dingle, Kate E; Sanderson, Nick; Brankin, Alice; Walker, Timothy; Sharma, Srilakshmi; Leong, James; Plaha, Puneet; Hofer, Monika; Chiodini, Peter; Gottstein, B; Furrer, Lavinia; Crook, Derrick; Brent, Andrew
• Is Part Of: Open forum infectious diseases. Volume 9:Number 9(2022)
• Publication Date: 2022-08-23
• Subjects: leprosy -- coenurosis -- Taenia -- mycobacteria -- whole genome sequencing; Communicable diseases -- Periodicals; Medical microbiology -- Periodicals; Infection -- Periodicals; 616.9
• DOI: 10.1093/ofid/ofac428
• Languages: English
• ISSNs: 2328-8957

Abstract

Abstract: Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts.