Tracking the Dynamic Folding and Unfolding of RNA G‐Quadruplexes in Live Cells. (13th March 2018)

• Record Type: Journal Article
• Authors: Chen, Xiu‐Cai; Chen, Shuo‐Bin; Dai, Jing; Yuan, Jia‐Hao; Ou, Tian‐Miao; Huang, Zhi‐Shu; Tan, Jia‐Heng
• Is Part Of: Angewandte Chemie. Volume 130:Number 17(2018)
• Page Start: 4792
• Page End: 4796
• Publication Date: 2018-03-13
• Subjects: Bildgebung lebender Zellen -- Faltung/Entfaltung -- Fluoreszenzsonden -- G-Quadruplexe -- RNA-Strukturen; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201801999
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Because of the absence of methods for tracking RNA G‐quadruplex dynamics, especially the folding and unfolding of this attractive structure in live cells, understanding of the biological roles of RNA G‐quadruplexes is so far limited. Herein, we report a new red‐emitting fluorescent probe, QUMA‐1, for the selective, continuous, and real‐time visualization of RNA G‐quadruplexes in live cells. The applications of QUMA‐1 in several previously intractable applications, including live‐cell imaging of the dynamic folding, unfolding, and movement of RNA G‐quadruplexes and the visualization of the unwinding of RNA G‐quadruplexes by RNA helicase have been demonstrated. Notably, our real‐time results revealed the complexity of the dynamics of RNA G‐quadruplexes in live cells. We anticipate that the further application of QUMA‐1 in combination with appropriate biological and imaging methods to explore the dynamics of RNA G‐quadruplexes will uncover more information about the biological roles of RNA G‐quadruplexes. Abstract : Die geheime Dynamik der RNA : Die Fluoreszenzsonde QUMA‐1 für die selektive und kontinuierliche Echtzeit‐Bildgebung von RNA‐G‐Quadruplexen in lebenden Zellen wird vorgestellt. Mithilfe von QUMA‐1 kann die RNA‐G‐Quadruplex‐Dynamik untersucht und die Entfaltung von RNA‐G‐Quadruplexen durch RNA‐Helikase visualisiert werden.