Separation of Peptides on HALO 2‐Micron Particles. (1st August 2016)

• Record Type: Journal Article
• Authors: Mant, Colin T.; Hodges, Robert S.
• Editors: Coligan, John E.; Dunn, Ben M.; Speicher, David W.; Wingfield, Paul T.
• Is Part Of: Current protocols in protein science. Volume 85(2016)
• Page Start: 11.6.1
• Page End: 11.6.16
• Publication Date: 2016-08-01
• Subjects: reversed‐phase HPLC -- separation of peptides -- solid core -- core‐shell -- fused‐core particles -- 2.0 micron particles -- trifluoroacetic acid (TFA) -- anionic ion‐pairing reagent; Proteins -- Laboratory manuals; Proteins; Clinical Laboratory Techniques; Genetic Techniques; Immunologic Techniques; Proteins; Laboratory manuals; 572.6028
• DOI: 10.1002/cpps.12
• Languages: English
• ISSNs: 1934-3655

Abstract

Abstract: Reversed‐phase high‐performance liquid chromatography (RP‐HPLC) is of fundamental importance to the isolation and separation of peptides, proteins, and other biomolecules. Hence, there is a continuing high demand for the development of RP‐HPLC stationary‐phase materials with enhanced separation efficiency. HALO packing materials began the revolution in "core‐shell" technology with the advantages of faster separations, higher resolution and peak capacity, high temperature stability, and rugged reliable performance compared to traditional HPLC and UHPLC. These materials are characterized by a solid core surrounded by a thin layer of porous material, and represent a technology for the future with continuing refinements. Such refinements are aided via the use of designed synthetic peptide standards during stationary‐phase development. Concomitantly, such standards also enable the researcher to monitor RP‐HPLC column performance and develop optimized separation protocols for peptides from a wide array of sources. © 2016 by John Wiley & Sons, Inc.