Anti-Helicobacter pylori, anti-Inflammatory, and Antioxidant Activities of Trunk Bark of Alstonia boonei (Apocynaceae). (15th September 2022)

• Record Type: Journal Article
• Authors: Fagni Njoya, Zenab Linda; Mbiantcha, Marius; Djuichou Nguemnang, Stephanie Flore; Matah Marthe, Vanessa Mba; Yousseu Nana, William; Madjo Kouam, Yacine Karelle; Ngoufack Azanze, Elvira; Tsafack, Eric Gonzal; Ateufack, Gilbert
• Other Names: Husain Kazim Academic Editor.
• Is Part Of: BioMed research international. Volume 2022(2022)
• Publication Date: 2022-09-15
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2022/9022135
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : An ulcer is an erosion of the gastric mucosa that occurs following an imbalance between the aggression and protective factors and/or an infection with Helicobacter pylori ( H. pylori ). About 90-100% of duodenal ulcers and 70-80% of gastric ulcers are caused by H. pylori . The objective of this work was to evaluate in vitro the anti- H. pylori activity and then the anti-inflammatory and antioxidant properties of aqueous and methanol extracts of Alstonia boonei . The anti- H. pylori tests (CMI and antiureasic activity) were determined using the agar well diffusion method, the microbroth dilution method, and the measurement of ammonia production by the indophenol method; the anti-inflammatory properties were evaluated by inhibition of proteinases, denaturation of albumin, production of NO by macrophages, cell viability, and hemolysis of red blood cells by heat; then, the antioxidant properties were evaluated by the FRAP method (ferric reducing antioxidant power) and the DPPH (1, 1-diphenyl-2-picrylhydrazyl) test. The results show that the best trapping of the DPPH radical was obtained with the methanol extract (EC50 = 8.91 μ g/mL) compared to the aqueous extract (EC50 = 19.86 μ g/mL). The methanol extract also showed greater iron-reducing activity than the aqueous extract and vitamin C. Furthermore, at the concentration of 200 μ g/mL, the methanol extract showed a percentage (96.34%) strains of H. pylori higher than that of the aqueous extract (88.52%). The MIC90 of the methanol extract was lower than that of the aqueous extract. The methanol extract showed a higher percentage inhibition (85%) of urease than the aqueous extract (73%). The methanol extract at a concentration of 1000 μ g/mL showed the greatest ability to inhibit proteinase activity, albumin denaturation, and red blood cell hemolysis; on the other hand, maximum cell viability and greater production of nitrite oxide by macrophages were obtained with the aqueous extract. Aqueous and methanol extracts of Alstonia boonei possess anti- H. pylori which would probably be linked to their antioxidant and anti-inflammatory properties.