Oral Human Papillomavirus Associated With Differences in Oral Microbiota Beta Diversity and Microbiota Abundance . (17th January 2022)

• Record Type: Journal Article
• Authors: Zhang, Yuehan; D'Souza, Gypsyamber; Fakhry, Carole; Bigelow, Elaine O; Usyk, Mykhaylo; Burk, Robert D; Zhao, Ni
• Is Part Of: Journal of infectious diseases. Volume 226:Number 6(2022)
• Page Start: 1098
• Page End: 1108
• Publication Date: 2022-01-17
• Subjects: functional pathways -- oral HPV -- oral microbiome; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiac010
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Background: Although cervicovaginal microbiome has been associated with cervical human papillomavirus (HPV) infection, little is known regarding the association of oral microbiome with oral HPV, a cause of oropharyngeal cancer. Methods: A cross-sectional analysis of 495 participants from the Men and Women Offering Understanding of Throat HPV study was conducted. 16S rRNA gene amplicon sequencing was performed on saliva samples. HPV DNA in oral rinse samples was tested. Associations of oral microbiome diversity, taxon abundance, and predicted functional pathways with oral HPV were assessed, adjusting for age, race/ethnicity, education, human immunodeficiency virus, current smoking, and sequencing batch. Results: Participants with oral HPV (n = 68) compared with those without HPV had similar oral microbiome alpha-diversity yet different beta-diversity (Bray-Curtis distance for bacterial taxa, P = .009; functional pathways, P = .02). Participants with oral HPV had higher abundance of Actinomycetaceae, Prevotellaceae, Veillonellaceae, Campylobacteraceae, Bacteroidetes, and lower abundance of Gemellaceae (false discovery rate <0.10). We also found differential functional potential of oral microbiome by oral HPV status: xenobiotic biodegradation-related pathways were less abundant among participants with oral HPV, suggesting potential xenobiotic-induced toxic effects with implications for HPV susceptibility. Conclusions: Our findings suggest a shift in oral microbiome community structure, composition, and functional potential between individuals with and without oral HPV. Abstract : Oral HPV was associated with differences in beta diversity of oral microbiome and higher abundance of Actinomycetaceae, Prevotellaceae, Veillonellaceae, Campylobacteraceae, Bacteroidetes, and lower abundance of Gemellacea. Predicted functional potential of oral microbiota was shifted among individuals with oral HPV.