Sustained vascular contractile response induced by an R- and S-epimer of the ergot alkaloid ergocristine and attenuation by a noncompetitive antagonist. (1st July 2022)

• Record Type: Journal Article
• Authors: Cherewyk, Jensen E; Parker, Sarah E; Blakley, Barry R; Al-Dissi, Ahmad N
• Is Part Of: Journal of animal science. Volume 100:Number 9(2022)
• Publication Date: 2022-07-01
• Subjects: artery -- Claviceps purpurea -- ergocristinine -- phenoxybenzamine; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/skac235
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: Vasoconstriction is a known effect associated with ergot alkaloid consumption. The vascular contractile responses are often sustained for an extended period after exposure. Ergot alkaloids exist in two molecular configurations, the C-8-( R )-isomer ( R -epimer) and the C-8-( S )-isomer ( S -epimer). The sustained vascular contractile response to the R -epimers has been studied previously, unlike the S -epimers which are thought to be biologically inactive. Additionally, antagonists have been utilized to attenuate the vascular contraction associated with the R- epimers of ergot alkaloids utilizing ex vivo techniques. This study utilized an arterial tissue bath to examine and compare the sustained vascular contractile response attributed to ergocristine ( R ) and ergocristinine ( S ) using dissected bovine metatarsal arteries. The contractile blocking effect of a noncompetitive alpha-adrenergic antagonist, phenoxybenzamine (POB), was also investigated in precontracted arteries. Arteries ( n = 6/epimer) were exposed to a single dose of ergocristine or ergocristinine (1 × 10 −6 M in buffer). Each of the epimer doses was followed by a POB (1 × 10 −3 M) or methanol (control) treatment at 90 min and the response was observed for another 90 min. Both epimers produced a sustained contractile response over the 180-min incubation period in the control groups. The R -epimer caused a greater sustained contractile response from 60 to 180 min post epimer exposure, compared to the S -epimer ( P < 0.05, generalized estimating equations, independent t-test). Phenoxybenzamine caused a decrease in the contractile response induced by ergocristine and ergocristinine from 105 to 180 min, compared to the control ( P < 0.05, generalized estimating equations, paired t-test). Overall, these results demonstrate the presence of a sustained vascular contractile response attributed to the R- and S -epimer of an ergot alkaloid with differences in contractile response between the epimers, suggesting differences in receptor binding mechanisms. Furthermore, this study demonstrated that a noncompetitive antagonist could attenuate the sustained arterial contractile effects of both ergot configurations ex vivo. Additional investigation into S -epimers of ergot alkaloids is needed. This research contributes to the understanding of the ergot epimer-vascular receptor binding mechanisms, which may support the investigation of different approaches of minimizing ergot toxicity in livestock. Abstract : The R- and S -epimer of an ergot alkaloid, ergocristine, and ergocristinine, produced a sustained vascular contractile response. A noncompetitive antagonist, phenoxybenzamine, alleviated vascular contraction during the sustained contractile response. Lay Summary: Ergot alkaloids cause blood vessels to contract when contaminated feed is consumed by animals. Vascular contraction often remains for a prolonged period and involves the binding of ergot to specific receptors in the blood vessels. This study assessed and compared the sustained contraction of cow arteries after exposure to two forms of an ergot alkaloid, namely, ergocristine and ergocristinine. The effects of a specific receptor blocker, phenoxybenzamine, on the vascular contraction induced by these forms were also examined. This study showed that both forms of ergot caused a sustained contraction of cow arteries but to different magnitudes. Differences in contraction could be related to differences in how each form of ergot binds to receptors. The receptor blocker decreased the sustained contractile response of both forms of ergot. Further understanding of how the different forms of ergot bind to receptors, and how to decrease the adverse effects, may help mitigate the toxic effects of ergotism.