Association of forkhead box P3 gene polymorphisms with premature ovarian insufficiency in Chinese women. (4th March 2021)

• Record Type: Journal Article
• Authors: Zhong, Chenyi; Wang, Wanyue; Pu, Danhua; Wang, Huiyuan; Tan, Rongrong; Wu, Jie
• Is Part Of: Gynecological endocrinology. Volume 37:Number 3(2021)
• Page Start: 246
• Page End: 250
• Publication Date: 2021-03-04
• Subjects: Premature ovarian insufficiency -- FOXP3 -- polymorphism -- complete gene sequences -- long-range polymerase chain reaction; Endocrine gynecology -- Periodicals; Generative organs, Female -- Diseases -- Periodicals; 618.1
• DOI: 10.1080/09513590.2020.1854716
• Languages: English
• ISSNs: 0951-3590

Abstract

Abstract: Objective: Forkhead box P3 (FOXP3), a transcription factor, is regarding critical regulator of the function of regulatory T (Treg) cells and plays a crucial role in the development of autoimmune diseases. Premature ovarian insufficiency (POI) is an autoimmune disease; however, little is known about the association between FOXP3 variants and the susceptibility to POI. Methods: Long-range polymerase chain reaction was used to analyze complete FOXP3 gene sequences from 153 patients with POI. The frequencies of genotypes and alleles of the FOXP3 gene were compared between patients with POI and 269 East Asian women from the Genome Aggregation (gnomAD) database. Results: Forty-three single-nucleotide polymorphisms (SNPs) were detected, including 25 known SNPs and 18 novel SNPs. The genotype distributions and allele frequencies of two known SNPs (rs17847094 and rs76798919) and three novel SNPs (NC_000023.11:g.49112832G > A, NC_000023.11:g.49112833G > A, and NC_000023.11:g.49120479CT > C) were significantly different between the two groups. Linkage disequilibrium and haplotype analyses of the rs57734889, rs2232365, rs3761548, and rs34629506 SNPs in FOXP3 were performed and compared, and the high D ′ (standardized disequilibrium coefficients) value indicated that these polymorphisms may contribute to the risk of POI. Conclusions: This study is the first to show that genetic variants in the regulatory regions of FOXP3 play a vital role in idiopathic POI in the Chinese population.