Copolymeric micelles as efficient inert nanocarrier for hypericin in the photodynamic inactivation of Candida species. (29th April 2019)

• Record Type: Journal Article
• Authors: Sakita, Karina M; Conrado, Pollyanna CV; Faria, Daniella R; Arita, Glaucia S; Capoci, Isis RG; Rodrigues-Vendramini, Franciele AV; Pieralisi, Neli; Cesar, Gabriel B; Gonçalves, Renato S; Caetano, Wilker; Hioka, Noboru; Kioshima, Erika S; Svidzinski, Terezinha IE; Bonfim-Mendonça, Patricia S
• Is Part Of: Future microbiology. Volume 14:Number 6(2019)
• Page Start: 519
• Page End: 531
• Publication Date: 2019-04-29
• Subjects: biofilm -- Candida spp. -- hypericin -- photodynamic inactivation -- resistance; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0304
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To evaluate the efficacy of photodynamic inactivation (PDI) mediated by hypericin encapsulated in P-123 copolymeric micelles (P123-Hyp) alone and in combination with fluconazole (FLU) against planktonic cells and biofilm formation of Candida speciesMaterials & methods: PDI was performed using P123-Hyp and an LED device with irradiance of 3.0 mW/cm 2 .Results: Most of isolates (70%) were completely inhibited with concentrations up to 2.0 μmol/l of HYP and light fluence of 16.2 J/cm 2 . FLU-resistant strains had synergic effect with P123-HYP-PDI and FLU. The biofilm formation was inhibited in all species, in additional the changes in Candida morphology observed by scanning electron microscopy.Conclusion: P123-Hyp-PDI is a promising option to treat fungal infections and medical devices to prevent biofilm formation and fungal spread.