Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids. Issue 10 (29th April 2022)

• Record Type: Journal Article
• Authors: Iribarren, Cristina; Nordlander, Sofia; Sundin, Johanna; Isaksson, Stefan; Savolainen, Otto; Törnblom, Hans; Magnusson, Maria K.; Simrén, Magnus; Öhman, Lena
• Is Part Of: Neurogastroenterology & motility. Volume 34:Issue 10(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-29
• Subjects: colonoids -- epithelial barrier -- host‐microbiota cross talk -- intestinal microenvironment -- irritable bowel syndrome; Gastrointestinal system -- Motility -- Periodicals; Gastrointestinal system -- Innervation -- Periodicals; 616.33
• DOI: 10.1111/nmo.14390
• Languages: English
• ISSNs: 1350-1925

Abstract

Abstract: Background: Alteration of the host‐microbiota cross talk at the intestinal barrier may participate in the pathophysiology of irritable bowel syndrome (IBS). Therefore, we aimed to determine effects of fecal luminal factors from IBS patients on the colonic epithelium using colonoids. Methods: Colon‐derived organoid monolayers, colonoids, generated from a healthy subject, underwent stimulation with fecal supernatants from healthy subjects and IBS patients with predominant diarrhea, phosphate‐buffered saline (PBS), or lipopolysaccharide (LPS). Cytokines in cell cultures and fecal LPS were measured by ELISA and mRNA gene expression of monolayers was analyzed using Qiagen RT 2 Profiler PCR Arrays. The fecal microbiota profile was determined by the GA‐map ™ dysbiosis test and the fecal metabolite profile was analyzed by untargeted liquid chromatography/mass spectrometry. Key results: Colonoid monolayers stimulated with fecal supernatants from healthy subjects ( n = 7), PBS ( n = 4) or LPS ( n = 3) presented distinct gene expression profiles, with some overlap (R 2 Y = 0.70, Q 2 = 0.43). Addition of fecal supernatants from healthy subjects and IBS patients ( n = 9) gave rise to different gene expression profiles of the colonoid monolayers (R 2 Y = 0.79, Q 2 = 0.64). Genes ( n = 22) related to immune response (CD1D, TLR5) and barrier integrity (CLDN15, DSC2) contributed to the separation. Levels of proinflammatory cytokines in colonoid monolayer cultures were comparable when stimulated with fecal supernatants from either donor types. Fecal microbiota and metabolite profiles, but not LPS content, differed between the study groups. Conclusions: Fecal luminal factors from IBS patients induce a distinct colonic epithelial gene expression, potentially reflecting the disease pathophysiology. The culture of colonoids from healthy subjects with fecal supernatants from IBS patients may facilitate the exploration of IBS related intestinal micro‐environmental and barrier interactions. Abstract : Colonoids from healthy subject cultured with fecal supernatants from IBS patients may facilitate the exploration of disease‐related intestinal micro‐environmental and barrier interactions.