Detection of African swine fever virus utilizing the portable MatMaCorp ASF detection system. Issue 5 (21st December 2021)

• Record Type: Journal Article
• Authors: Zurita, Mariceny; Martignette, Lauren; Barrera, Jose; Carrie, Michael; Piscatelli, Heather; Hangman, Alyssa; Brake, David; Neilan, John; Petrik, Dustin; Puckette, Michael
• Is Part Of: Transboundary and emerging diseases. Volume 69:Issue 5(2022)
• Page Start: 2600
• Page End: 2608
• Publication Date: 2021-12-21
• Subjects: African swine fever -- amplification -- detection -- food safety -- Solas 8 -- tissues; Veterinary medicine -- Periodicals; 636.089
• DOI: 10.1111/tbed.14411
• Languages: English
• ISSNs: 1865-1674

Abstract

Abstract: African swine fever (ASF) has emerged as a major threat to domestic and wild suid populations, and its continued spread threatens commercial swine production worldwide. The causative agent of ASF, African swine fever virus (ASFV), possesses a linear, double stranded DNA genome. Traditional detection of ASFV relies on laboratory‐based virus isolation or real‐time PCR of samples, typically blood or spleen, obtained from suspect cases. While effective, these methodologies are not easily field deployable, a major limitation during disease outbreak and response management scenarios. In this report, we evaluated the MatMaCorp Solas 8 ® ASFV detection system, a field deployable DNA extraction and fluorescent detection device, for its ability to extract and detect ASFV from multiple sample types obtained from domestic swine experimentally infected with ASFV strain Georgia. We found that the MatMaCorp Solas 8 ® ASFV detection device, and affiliated MagicTip™ DNA extraction and C‐SAND™ assay kits, readily detected ASFV in blood and spleen, as well as other sample types, including pinna, liver, skin, muscle and bone marrow.