Characterizing the genetic basis of trait evolution in the Mexican cavefish. Issue 5 (4th August 2022)

• Record Type: Journal Article
• Authors: Oliva, Camila; Hinz, Nicole K.; Robinson, Wayne; Barrett Thompson, Alexys M.; Booth, Julianna; Crisostomo, Lina M.; Zanineli, Samantha; Tanner, Maureen; Lloyd, Evan; O'Gorman, Morgan; McDole, Brittnee; Paz, Alexandra; Kozol, Rob; Brown, Elizabeth B.; Kowalko, Johanna E.; Fily, Yaouen; Duboue, Erik R.; Keene, Alex C.
• Is Part Of: Evolution & development. Volume 24:Issue 5(2022)
• Page Start: 131
• Page End: 144
• Publication Date: 2022-08-04
• Subjects: cavefish -- development -- behavior; Evolution (Biology) -- Periodicals; Developmental biology -- Periodicals; 576.82
• DOI: 10.1111/ede.12412
• Languages: English
• ISSNs: 1520-541X

Abstract

Abstract: Evolution in response to a change in ecology often coincides with various morphological, physiological, and behavioral traits. For most organisms little is known about the genetic and functional relationship between evolutionarily derived traits, representing a critical gap in our understanding of adaptation. The Mexican tetra, Astyanax mexicanus, consists of largely independent populations of fish that inhabit at least 30 caves in Northeast Mexico, and a surface fish population, that inhabit the rivers of Mexico and Southern Texas. The recent application of molecular genetic approaches combined with behavioral phenotyping have established A . mexicanus as a model for studying the evolution of complex traits. Cave populations of A . mexicanus are interfertile with surface populations and have evolved numerous traits including eye degeneration, insomnia, albinism, and enhanced mechanosensory function. The interfertility of different populations from the same species provides a unique opportunity to define the genetic relationship between evolved traits and assess the co‐evolution of behavioral and morphological traits with one another. To define the relationships between morphological and behavioral traits, we developed a pipeline to test individual fish for multiple traits. This pipeline confirmed differences in locomotor activity, prey capture, and startle reflex between surface and cavefish populations. To measure the relationship between traits, individual F2 hybrid fish were characterized for locomotor behavior, prey‐capture behavior, startle reflex, and morphological attributes. Analysis revealed an association between body length and slower escape reflex, suggesting a trade‐off between increased size and predator avoidance in cavefish. Overall, there were few associations between individual behavioral traits, or behavioral and morphological traits, suggesting independent genetic changes underlie the evolution of the measured behavioral and morphological traits. Taken together, this approach provides a novel system to identify genetic underpinnings of naturally occurring variation in morphological and behavioral traits. Abstract : Individual hybrid fish (top) were assayed for various behaviors (bottom) revealing genetic relationships between behaviors. Research highlights: 1) We examined the relationship between evolved morphological traits in the cave‐dwelling, form and the behavioral changes the cave form exhibits. 2) We find that shared genetic architecture regulates numerous traits associated with body and size. 3) We identified behavioral and morphological traits are largely regulated by independent genetic architecture. 4) These experiments provide a framework for future studies that apply genetic mapping to identify genetic architecture regulating complex traits.