Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis. Issue 18 (8th September 2022)

• Record Type: Journal Article
• Authors: Jing, Yongshuai; Zhang, Ruijuan; Ma, Yunfeng; Zhang, Yuwei; Zheng, Yuguang; Wu, Lanfang; Zhang, Danshen
• Is Part Of: Natural product research. Volume 36:Issue 18(2022)
• Page Start: 4630
• Page End: 4635
• Publication Date: 2022-09-08
• Subjects: Glehnia littoralis -- polysaccharide -- structural elucidation -- anti-radical activity -- immunomodulatory activity; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2021.2007095
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis . A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained. The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction. The molecular weight distributions of GLP were determined as 1.89 × 10 6 and 1.26 × 10 4 Da. The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively. The average molecular weight of GLP80-1 was determined as 1.63 × 10 4 Da. The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6. The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells. The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food. Graphical Abstract: UF0001