Optimization of enzymatic parameters for the production of formononetin from red clover (Trifolium pratense L.) through a response surface methodology. Issue 18 (8th September 2022)

• Record Type: Journal Article
• Authors: Muñoz, María Teresa; Maldonado, Vanessa; Herrera, Wence; Mutis, Ana; Bardehle, Leonardo; Medina, Cristian; Hormazábal, Emilio; Ortega, Fernando; Quiroz, Andrés
• Is Part Of: Natural product research. Volume 36:Issue 18(2022)
• Page Start: 4719
• Page End: 4724
• Publication Date: 2022-09-08
• Subjects: Red clover (Trifolium pratense L.) -- formononetin -- enzymatic assay -- isoflavone synthase; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2021.2002324
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Trifolium pratense, is a forage found worldwide, but it is negatively impacted by the clover root borer, Hylastinus obscurus . Methanolic extraction has been reported for isolating formononetin from vegetal tissues, with an antifeeding effect on H. obscurus . However, this methodology is time-consuming and also extracts other secondary metabolites, whereas enzymatic assays can provide higher specificity. Hence, the objective of this work was to determine the optimal conditions in pH, temperature, and incubation time for the activity of isoflavone synthase via a response surface model. Once these parameters were optimized, the concentration of formononetin in cultivars and experimental lines of T. pratense was evaluated enzymatically. The results showed that the best condition for developing the enzymatic assay was pH 9.1 with an incubation at 34.5 °C for 155 min. The formononetin content fluctuated between 0.74 and 1.96 mg/g of fresh weight, where Precoz-3, Precoz-1, and Superqueli-INIA presented the highest production. Graphical Abstract: UF0001