The effects of microRNA-126 reduced inflammation and apoptosis of diabetic nephropathy through PI3K/AKT signalling pathway by VEGF. (3rd September 2022)

• Record Type: Journal Article
• Authors: Lou, Zhe; Li, Qiaobei; Wang, Chunyan; Li, Yinyan
• Is Part Of: Archives of physiology and biochemistry. Volume 128:Number 5(2022)
• Page Start: 1265
• Page End: 1274
• Publication Date: 2022-09-03
• Subjects: MicroRNA-126 -- diabetic nephropathy -- VEGF -- PI3K -- AKT; Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.1080/13813455.2020.1767146
• Languages: English
• ISSNs: 1381-3455

Abstract

Abstract: Gene expression microarray and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to measure the expression of miR-126. In model of diabetic nephropathy, we demonstrated that miR-126 expression was down-regulated, compared with control group. Down-expression of miR-126 promoted cell apoptosis and increased inflammation (as indicated by the levels of IL-1β, IL-6, IL-18 and TNF-α) of diabetic nephropathy in vitro . miR-126 over-expression led to significant inhibition of cell apoptosis and suppressed inflammation (IL-1β, IL-6, IL-18 and TNF-α). However, the down-expression of miR-126 suppressed the protein expression of VEGF, PI3K and p-AKT in diabetic nephropathy in vitro . On the contrary, over-expression of miR-126 induced the protein expression of VEGF, PI3K and p-AKT in diabetic nephropathy in vitro . The inhibition of VEGF increased the effect of miR-126 down-expression on apoptosis and inflammation in diabetic nephropathy in vitro . We investigated the specific function of miR-126 in patients with diabetic nephropathy and its possible mechanism.