Attachment of Nucleosides and Other Linkers to Solid‐Phase Supports for Oligonucleotide Synthesis. Issue 1 (1st March 2013)

• Record Type: Journal Article
• Authors: Guzaev, Andrei P.; Pon, Richard T.
• Editors: Beaucage, Serge L.; Bergstrom, Donald E.; Herdewijn, Piet; Matsuda, Akira
• Is Part Of: Current protocols in nucleic acid chemistry. Volume 52:Issue 1(2013)
• Page Start: 3.2.1
• Page End: 3.2.23
• Publication Date: 2013-03-01
• Subjects: nucleic acid chemistry -- oligonucleotides -- nucleosides and nucleotides -- solid supports; Nucleic acids -- Laboratory manuals; Nucleic Acids -- chemical synthesis; Nucleic acids; Laboratory Manuals; Electronic books; Laboratory manuals; 572.8028
• DOI: 10.1002/0471142700.nc0302s52
• Languages: English
• ISSNs: 1934-9270

Abstract

Abstract: Specific step‐by‐step instructions for conversion of 5′‐ O ‐(4, 4′‐dimethoxytrityl)‐ and base‐protected nucleosides and other mono‐ O ‐(4, 4′‐dimethoxytrityl)‐protected diols to their hemisuccinate esters and their coupling to CPG (controlled‐pore glass) supports bearing aminopropyl or long chain aminoalkyl groups are presented. Additional guidelines are provided for selecting a coupling protocol and performing in‐process control. Curr. Protoc. Nucleic Acid Chem . 52:3.2.1‐3.2.23. © 2013 by John Wiley & Sons, Inc.