AAV9 Delivery of shRNA to the Mouse Heart. Issue 1 (1st July 2016)

• Record Type: Journal Article
• Authors: Wakimoto, Hiroko; Seidman, J.G.; Foo, Roger S.Y.; Jiang, Jianming
• Editors: Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin
• Is Part Of: Current protocols in molecular biology. Volume 115:Issue 1(2016)
• Page Start: 23.16.1
• Page End: 23.16.9
• Publication Date: 2016-07-01
• Subjects: AAV -- cardiovascular -- shRNA; Molecular biology -- Technique -- Periodicals; Molecular biology -- Laboratory manuals; Molecular Biology -- methods; Biologie moléculaire -- Technique; Biologie moléculaire -- Manuels de laboratoire; Molecular biology; Molecular biology -- Technique; Laboratory Manual; Electronic reference sources; Laboratory manuals; 572.8028
• DOI: 10.1002/cpmb.9
• Languages: English
• ISSNs: 1934-3639

Abstract

Abstract: RNA interference (RNAi) is a rapid approach to dissect loss‐of‐function phenotype for a gene of interest. However, it is challenging to perform RNAi in specific organs and tissues in vivo. Engineered viruses can provide a useful tool for delivery of small RNAs in vivo. Recombinant adeno‐associated viruses (rAAVs) are the preferred method for delivering genes or gene modulators to target cells due to their high titer, low immune response, ability to transduce many types of cell, and overall safety. In this unit, we describe protocols for use of rAAVs as a cargo to deliver miRNA backbone‐based shRNA controlled by a cardiac‐specific promoter into the mouse heart. © 2016 by John Wiley & Sons, Inc.