A Microbiome DNA Enrichment Method for Next‐Generation Sequencing Sample Preparation. Issue 1 (1st July 2016)

• Record Type: Journal Article
• Authors: Yigit, Erbay; Feehery, George R.; Langhorst, Bradley W.; Stewart, Fiona J.; Dimalanta, Eileen T.; Pradhan, Sriharsa; Slatko, Barton; Gardner, Andrew F.; McFarland, James; Sumner, Christine; Davis, Theodore B.
• Editors: Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin
• Is Part Of: Current protocols in molecular biology. Volume 115:Issue 1(2016)
• Page Start: 7.26.1
• Page End: 7.26.14
• Publication Date: 2016-07-01
• Subjects: DNA sequencing -- microbiome -- metagenomics DNA enrichment -- methylation -- next‐generation sequencing -- NGG -- target enrichment; Molecular biology -- Technique -- Periodicals; Molecular biology -- Laboratory manuals; Molecular Biology -- methods; Biologie moléculaire -- Technique; Biologie moléculaire -- Manuels de laboratoire; Molecular biology; Molecular biology -- Technique; Laboratory Manual; Electronic reference sources; Laboratory manuals; 572.8028
• DOI: 10.1002/cpmb.12
• Languages: English
• ISSNs: 1934-3639

Abstract

Abstract: "Microbiome" is used to describe the communities of microorganisms and their genes in a particular environment, including communities in association with a eukaryotic host or part of a host. One challenge in microbiome analysis concerns the presence of host DNA in samples. Removal of host DNA before sequencing results in greater sequence depth of the intended microbiome target population. This unit describes a novel method of microbial DNA enrichment in which methylated host DNA such as human genomic DNA is selectively bound and separated from microbial DNA before next‐generation sequencing (NGS) library construction. This microbiome enrichment technique yields a higher fraction of microbial sequencing reads and improved read quality resulting in a reduced cost of downstream data generation and analysis. © 2016 by John Wiley & Sons, Inc.