Preparation and characterization of young Prunus persica fruit fraction and its anti-inflammatory effect on a transgenic zebrafish model. Issue 19 (21st September 2022)

• Record Type: Journal Article
• Authors: Zhang, Xuanming; Wang, Lizhen; Li, Xiaobin; Zhang, Shanshan; Hou, Hairong; Zhang, Mengqi; He, Qiuxia; Liu, Kechun
• Is Part Of: Natural product research. Volume 36:Issue 19(2022)
• Page Start: 5048
• Page End: 5052
• Publication Date: 2022-09-21
• Subjects: Young Prunus persica fruits -- response surface methodology -- macroporous resins -- LC-MS/MS -- anti-inflammatory -- transgenic zebrafish; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2021.1912748
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Young Prunus persica fruits (YPF) contain substances that are distinct from those found in the mature fruits. Response surface methodology was used to explore the influences of extraction conditions including ultrasonic time (X1 ), ethanol proportion (X2 ), liquid-to-solid ratio (X3 ) and temperature (X4 ) on UV-absorbing components from YPF. To purify the extract, the adsorption/desorption properties of 280 nm-absorbing components on AB-8 resin were investigated. A total of 11 metabolites (amino acids, glycosylated amino acids and phenolics) were identified in the UV-absorbing fraction of YPF (YPF-F) based on LC-MS/MS assays. In a study of in vivo anti-inflammatory activity, YPF-F significantly decreased the number of inflammatory cells that migrated to the lateral line location in CuSO4 -induced transgenic fluorescent zebrafish. YPF should be utilized as a high value resource of functional foods.