Fundamentals of Acoustic Cytometry. Issue 1 (16th April 2018)

• Record Type: Journal Article
• Authors: Ward, Michael D.; Kaduchak, Gregory
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Dean, Phillip N.; Hibbs, Alan R.; Orfao, Alberto; Rabinovitch, Peter S.; Wheeless, Leon L.
• Is Part Of: Current protocols in cytometry. Volume 84:Issue 1(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-04-16
• Subjects: acoustic focus -- acoustic cytometry -- high‐speed flow cytometry -- hydrodynamic focus -- sample preparation; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/cpcy.36
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: Acoustic cytometry uses radiation pressure forces instead of or in addition to hydrodynamic focusing to position cells or particles in a flowing stream for analysis. Commercial implementations to date combine both hydrodynamic and acoustic focusing together to enable high precision analysis of a broad dynamic range of volumetric sample input rates up to an order of magnitude higher than is practical with hydrodynamic focus alone. This capability allows great flexibility in reducing assay time or modifying or eliminating concentration requirements or concentration steps in sample preparation protocols. It also provides a practical method for processing sub‐microliter volumes using sample dilution. In order to take full advantage of this dynamic range, it is necessary to understand the fundamental benefits and limitations of acoustic focusing as applied to flow cytometry. © 2018 by John Wiley & Sons, Inc.