Comparative global B cell receptor repertoire difference induced by SARS-CoV-2 infection or vaccination via single-cell V(D)J sequencing. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: He, Bing; Liu, Shuning; Xu, Mengxin; Hu, Yunqi; Lv, Kexin; Wang, Yuanyuan; Ma, Yong; Zhai, Yanmei; Yue, Xinyu; Liu, Lin; Lu, Hongjie; Zhou, Siwei; Li, Pengbin; Mai, Guoqin; Huang, Xiaoping; Li, Chenhang; Chen, Shifeng; Ye, Shupei; Zhao, Pingsen; Yang, Yuedong; Li, Xinhua; Jie, Yusheng; Shi, Mang; Yang, Jingyi; Shu, Yuelong; Chen, Yao-Qing
• Is Part Of: Emerging microbes & infections. Volume 11:Issue 1(2022)
• Page Start: 2007
• Page End: 2020
• Publication Date: 2022-12-31
• Subjects: BCR repertoire -- single-cell RNA sequencing -- COVID-19 -- SARS-CoV-2 vaccination -- SARS-CoV-2 infection -- inactivated vaccine; Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1080/22221751.2022.2105261
• Languages: English
• ISSNs: 2222-1751

Abstract

ABSTRACT: Dynamic changes of the paired heavy and light chain B cell receptor (BCR) repertoire provide an essential insight into understanding the humoral immune response post-SARS-CoV-2 infection and vaccination. However, differences between the endogenous paired BCR repertoire kinetics in SARS-CoV-2 infection and previously recovered/naïve subjects treated with the inactivated vaccine remain largely unknown. We performed single-cell V(D)J sequencing of B cells from six healthy donors with three shots of inactivated SARS-CoV-2 vaccine (BBIBP-CorV), five people who received the BBIBP-CorV vaccine after having recovered from COVID-19, five unvaccinated COVID-19 recovered patients and then integrated with public data of B cells from four SARS-CoV-2-infected subjects. We discovered that BCR variable (V) genes were more prominently used in the SARS-CoV-2 exposed groups (both in the group with active infection and in the group that had recovered) than in the vaccinated groups. The VH gene that expanded the most after SARS-CoV-2 infection was IGHV3-33, while IGHV3-23 in the vaccinated groups. SARS-CoV-2-infected group enhanced more BCR clonal expansion and somatic hypermutation than the vaccinated healthy group. A small proportion of public clonotypes were shared between the SARS-CoV-2 infected, vaccinated healthy, and recovered groups. Moreover, several public antibodies had been identified against SARS-CoV-2 spike protein. We comprehensively characterize the paired heavy and light chain BCR repertoire from SARS-CoV-2 infection to vaccination, providing further guidance for the development of the next-generation precision vaccine.