Surveillance for multidrug resistant Escherichia coli carriage in cattle, dogs and humans reveals predominance of CMY-2, CTX-M-15 and CTX-M-9 groups of β-lactamases. (October 2022)

• Record Type: Journal Article
• Authors: Ortiz, Daniel A.; Legenza, Laurel M.; Olson, Brooke J.; Knapp, Cindy C.; Killian, Scott B.; Meece, Jennifer K.; Hall, Matthew C.; Fritsche, Thomas R.
• Is Part Of: Comparative immunology, microbiology and infectious diseases. Volume 89(2022)
• Publication Date: 2022-10
• Subjects: E. coli, ESBL -- AmpC -- CRE -- DNA microarray -- Surveillance -- One Health; Communicable diseases in animals -- Periodicals; Veterinary immunology -- Periodicals; Veterinary microbiology -- Periodicals; Immunology -- Periodicals; Microbiology -- Periodicals; Communicable diseases -- Periodicals; Communicable Diseases -- immunology -- Periodicals; Communicable Diseases -- veterinary -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Veterinary Medicine -- Periodicals; Immunologie -- Périodiques; Microbiologie -- Périodiques; Maladies infectieuses -- Périodiques; Communicable diseases; Immunology; Microbiology; Electronic journals; Periodicals; 636.08969
• DOI: 10.1016/j.cimid.2022.101880
• Languages: English
• ISSNs: 0147-9571

Abstract

Abstract: Global spread of antimicrobial multidrug resistance (MDR) in human and veterinary medicine relies upon diagnostics, surveillance and stewardship to guide mitigation. Utilizing surveillance of fecal samples from our service area for detecting MDR Escherichia coli carriage in humans (2143), dogs (627), and cattle (130), we found isolates resistant to third/fourth generation cephems present in 3.7 %, 13.1 %, and 51.5 %, respectively. CMY-2, CTX-M-15-like and CTX-M9 group genes in descending order were predominant in all hosts and accounted for 83.3 % of non-wild-type gene targets. MDR carriage mirrored cephem non-susceptibility rates as published in annual antibiograms for humans and dogs; notably, no carbapenem-resistant carriage isolates were detected. Given the scale of MDR E. coli carriage in cattle (14X) and dogs (3.5X) compared to humans, bench-marking of the resistance gene pool by host species utilizing regional One Health surveillance may aid in assessing occupational and geographic risks for acquiring resistance and for monitoring of mitigation strategies. Highlights: One Health surveillance is a tool for documenting antimicrobial resistance emergence 87. Humans, dogs and cattle were screened for cephem-resistant E. coli carriage 77. Multi-drug resistance was detected in 3.7 %, 13.1 % and 51.5 % of humans, dogs and cattle 86. CMY-2, CTX-M15-like and CTX-M9 group genes were predominant in all hosts 74. Gene pool benchmarking may aid in assessing health risks and mitigation monitoring 84.