MicoRNA-214-3p: a key player in CPLX2-mediated inhibition on temozolomide resistance in glioma. (3rd October 2022)

• Record Type: Journal Article
• Authors: Peng, Qian; Wang, Lijiao; Wang, Shuling; Wang, Chenxu; Xue, Zhi
• Is Part Of: Neurological research. Volume 44:Number 10(2022)
• Page Start: 879
• Page End: 887
• Publication Date: 2022-10-03
• Subjects: Glioma -- temozolomide resistance -- miR-214-3p -- CPLX2 -- proliferation -- apoptosis; Neurology -- Periodicals; Neurosciences -- Periodicals; 616.8005
• DOI: 10.1080/01616412.2022.2064699
• Languages: English
• ISSNs: 0161-6412

Abstract

ABSTRACT: Objective: This study plans to investigate whether miR-214-3p could bind CPLX2 to regulate temozolomide (TMZ) resistance in glioma. Methods: The differential expression of miR-214-3p and CPLX2 was determined by qRT-PCR and Western blotting in TMZ-resistant glioma tissues. Then, TMZ-resistant glioma cells (U87/TMZ and U251/TMZ) were established and transfected with miR-214-3p mimic, miR-214-3p inhibitor, pcDNA3.1-CPLX2 or pcDNA3.1-CPLX2 plus miR-214-3p mimic to evaluate the impact of miR-214-3p and CPLX2 on the proliferation, apoptosis and TMZ resistance in U87/TMZ and U251/TMZ cells. The binding relationship between miR-214-3p and CPLX2 was reported by dual-luciferase reporter assay. Results: Higher miR-214-3p and lower CPLX2 expression levels were revealed in TMZ-sensitive glioma tissues. The alterations in miR-214-3p and CPLX2 expression were more significant in TMZ-resistant tissues compared with TMZ-sensitive tissues. In cellular experiments, TMZ-resistant cells expressed higher miR-214-3p expression and lower CPLX2 expression than TMZ-sensitive cells. Transfection of miR-214-3p mimic elevated the proliferation and half maximal inhibitory concentration (IC50 ) and decreased the apoptosis in U87/TMZ and U251/TMZ cells. Introduction of miR-214-3p inhibitor or pcDNA3.1-CPLX2 reduced the proliferation and IC50 value and prompted the apoptosis in TMZ-resistant glioma cells. The effects of pcDNA3.1-CPLX2 on inhibiting the proliferation and IC50 value and enhancing the apoptosis in TMZ-resistant glioma cells were hindered by the transfection of miR-214-3p mimic. In addition, CPLX2 was a target gene of miR-214-3p. Conclusion: Downregulation of miR-214-3p inhibits TMZ resistance in glioma by promoting CPLX2.