OMIP‐068: High‐Dimensional Characterization of Global and Antigen‐Specific B Cells in Chronic Infection. Issue 10 (28th August 2020)

• Record Type: Journal Article
• Authors: Cascino, Katherine; Roederer, Mario; Liechti, Thomas
• Is Part Of: Cytometry. Volume 97:Issue 10(2020)
• Page Start: 1037
• Page End: 1043
• Publication Date: 2020-08-28
• Subjects: B cells -- antigen‐specific B cells -- chronic Infections -- phenotyping -- memory B cells -- HBV; Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.24204
• Languages: English
• ISSNs: 1552-4922

Abstract

Abstract: This 24‐color flow cytometry panel focuses on characterizing antigen‐specific B cells and precise delineation of B‐cell subsets in chronic infections and is applicable to other chronic diseases such as autoimmunity. The panel was optimized for human cryopreserved peripheral blood mononuclear cells (PBMCs). Markers were chosen to extensively distinguish B‐cell lineages (CD19, CD20, CD10, CD38, CD24, IgM, IgD, CD27, CD21, CD43, CD5). Inclusion of antigen‐specific probes was of high priority in order to assess hepatitis B virus (HBV) antigen‐specific B cells for our purposes. These probes can be readily exchanged for other pathogen‐specific probes or additional markers for the panel to be tailored to desired research questions beyond HBV. In addition, we included a comprehensive and unique set of functional markers such as chemokine receptors (CXCR3, CXCR5), co‐stimulatory molecule (CD86), Fc receptor (CD32), regulatory molecules (BTLA, CD39), and inhibitory markers associated with chronic infections (PD‐1, FcRL5, CD11c, CD22) to enable in‐depth analysis of global and antigen‐specific B cells during chronic infection. © 2020 International Society for Advancement of Cytometry