AIEgens assisted label free DNA supersandwich immunoassay for ultrasensitive α-fetoprotein detection. (August 2022)

• Record Type: Journal Article
• Authors: Ou, Xiaowen; Dai, Jingman; Huang, Yiting; Xiong, Xiaoqin; Zheng, Zhi; Lou, Xiaoding; Xia, Fan
• Is Part Of: Giant. Volume 11(2022)
• Publication Date: 2022-08
• Subjects: AIEgens -- α-fetoprotein detection -- DNA supersandwich immunoassay -- High signal-to-background ratio -- Cancer diagnose; AIE aggregation-induced emission -- DSI DNA supersandwich immunoassay -- AFP α-fetoprotein -- AIEgens fluorogen with AIE feature -- FITC fluorescein isothiocyanate -- ssDNA single stranded DNA -- ACQ aggregation-caused quenching -- Ab1 primary antibody -- Ab2 secondary antibody -- PS polystyrene microspheres -- BSA bull serum albumin -- CEA carcino-embryonic antigen -- DD d-dimer -- HCG human chorionic gonadotropin -- PCT procalcitonin -- PSA prostate specific antigen -- IgE immunoglobulin E; Macromolecules -- Periodicals; Nanostructured materials -- Periodicals; Smart materials -- Periodicals; Biomimetic materials -- Periodicals; Nanostructures; Smart Materials; Biomimetic Materials; Macromolecular Substances; Biomimetic materials; Macromolecules; Nanostructured materials; Smart materials; Electronic journals; Periodical; Periodicals; 547.7
• DOI: 10.1016/j.giant.2022.100110
• Languages: English
• ISSNs: 2666-5425

Abstract

Abstract: The traditional enzyme-linked immunosorbent assay has been widely applied in the field of protein detection. Nevertheless, the detecting performance suffers from high background. Herein, we choose label free molecules of aggregation-induced emission (AIE) to design DNA supersandwich immunoassay (DSI) for ultrasensitive α-fetoprotein (AFP) detection. The silole-R, typical water soluble AIEgens (fluorogen with AIE feature) with positive charges, adsorbs on phosphate backbone of DSI by electrostatic attraction. In this way, the AIEgens DSI allows for ultrasensitive detecting AFP with low limit of detection (1.4 ng/mL). Moreover, the AIEgens DSI demonstrates a higher signal-to-background ratio (10.94) than fluorescein isothiocyanate (FITC) DSI (4.38). This AIEgens DSI provides a novel fluorescent enhanced analysis of trace AFP for cancer diagnose. Graphical abstract: Image, graphical abstract .