Impaired Th17 cell proliferation and decreased pro‐inflammatory cytokine production in CXCR3/CXCR4 double‐deficient mice of vulvovaginal candidiasis. Issue 8 (17th January 2019)

• Record Type: Journal Article
• Authors: Jin, Yue‐Mei; Liu, Shan‐Shan; Xu, Tian‐Min; Guo, Feng‐Jun; Chen, Jun
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 8(2019:Aug.)
• Page Start: 13894
• Page End: 13905
• Publication Date: 2019-01-17
• Subjects: CXCR3 -- CXCR4 -- gene silencing -- inflammatory factors -- Th17 cells -- vulvovaginal candidiasis (VVC); Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28071
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Vulvovaginal candidiasis (VVC) is a common observed infection, affecting approximately 75% of women of reproductive age. Drug resistance represents a troublesome stumbling block associated with VVC therapy. Thus the aim of the present study was to provide information regarding the selection of potential drug targets for VVC. CXCR3‐, CXCR4‐, or CXCR/CXCR4 double‐deficient mouse models of VVC were subsequently established, with changes to the load of Candida Albicans evaluated accordingly. The biological behaviors of the vaginal epithelial cells were characterized in response to the CXCR3‐, CXCR4‐, or CXCR3/CXCR4 double‐knockout in vivo. Our initial observations revealed that in mice with VVC, CXCR3‐, CXCR4‐, or CXCR3 ‐ CXCR4 double‐knockout resulted in a decreased load of C. Albicans as well as reduced levels and proportion of Th17 cells. Proinflammatory cytokine production was found to be inhibited by CXCR3‐, CXCR4‐, or CXCR3/CXCR4 double‐knockout whereby the mRNA and protein expressions CXCR3, CXCR4, IL‐17, IL‐6, and TNF‐α exhibited decreased levels. CXCR3‐, CXCR4‐, or CXCR3/CXCR4 double‐knockout appeared to function as positive proliferation factors, while playing a negative role in the processes of apoptosis and the cell cycle of vaginal epithelial cells. Taken together, the key findings of the study suggested that CXCR3/CXCR4 double‐knockout could act to hinder the progression of VVC, highlighting its promise as a novel therapeutic target in the treatment of VVC. CXCR3 and CXCR4 genes may regulate Th17/IL‐17 immune inflammatory pathways to participate in antifungal immunity. Abstract : Taken together, the key findings of the study suggested that CXCR3/CXCR4 double‐knockout could act to hinder the progression of vulvovaginal candidiasis (VVC), highlighting its promise as a novel therapeutic target in the treatment of VVC. CXCR3 and CXCR4 genes may regulate Th17/IL‐17 immune inflammatory pathways to participate in antifungal immunity.