Growth performance and intestinal microbial changes of Carassius auratus in response to pomegranate (Punica granatum) peel extract‐supplemented diets. Issue 4 (13th December 2020)

• Record Type: Journal Article
• Authors: Ahmadniaye Motlagh, Hamidreza; Rokhnareh, Zahra; Safari, Omid; Selahvarzi, Yahya
• Is Part Of: Journal of the World Aquaculture Society. Volume 52:Issue 4(2021)
• Page Start: 820
• Page End: 828
• Publication Date: 2020-12-13
• Subjects: aquaculture -- flavonoid -- intestinal microbiota -- phenol -- pomegranate; Aquaculture -- Periodicals; 639.8
• DOI: 10.1111/jwas.12754
• Languages: English
• ISSNs: 0893-8849

Abstract

Abstract: This study aimed to investigate the effect of pomegranate ( Punica granatum ) peel extract (PPE) on growth indices, intestinal bacteria, and total fungi count of Carassius auratus intestine. Ninety C. auratus fries (11.04 ± 0.22 g) were distributed among 15 aquariums (100‐L) in a completely randomized design (triplicated), and were fed diets containing 0.1, 1, 2, and 4% PPE for 60 days at a feeding rate of 2% body weight during the experiment. A spectrophotometry assessment indicated that the total phenol and flavonoid content of PPE was 5.44 ± 0.03 and 49.80 ± 1.00 mg/g, respectively. According to the results, the extract significantly reduced the growth indices (final weight, weight gain, specific growth rate (SGR), and feed conversion ratio (FCR)) at 4% compared to the control ( p < .05). PPE did not affect the total aerobic bacteria or total lactic acid bacteria count, but the enteric gram‐negative bacteria count was significantly reduced in the experimental treatments ( p < .05). The total fungi count showed a significant increase in all treated fish ( p < .05). According to the results, because of the negative effect of the high dose of PPE on growth, there is a recommendation for it to be used at 2% of the diet to reduce intestinal gram‐negative bacteria and increase the total fungi count.