Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora. (October 2022)

• Record Type: Journal Article
• Authors: Yu, Jincheng; Jiang, Wenhong; Wang, Shuyu; Liu, Suwen; Shi, Donglin; Wang, Hao; Chang, Xuedong
• Is Part Of: Journal of functional foods. Volume 97(2022)
• Publication Date: 2022-10
• Subjects: Hawthorn polyphenol -- Microcapsule -- Anti-fatigue -- Intestinal microflora -- Inflammation; AKT protein kinase B -- AMP adenosine monophosphate -- AMPK adenosine monophosphate-activated protein kinase -- BLA blood lactate -- BUN serum urea nitrogen -- CaMKKβ calcium-calmodulin kinase 2 -- CK creatine kinase -- CNTF ciliary neurotrophic factor -- GA gum arabic -- GDF8 growth differentiation factor 8 -- GSH glutathione -- GSK-3β glycogen synthase kinase-3β -- H&E hematoxylin and eosin -- HP hawthorn polyphenol -- HPM hawthorn polyphenol microcapsule -- IL-1β interleukin-1β -- IL-6 interleukin-6 -- IRS infrared spectrum -- LC l-carnitine -- LDA linear discriminant analysis -- LDH lactate dehydrogenase -- LG liver glycogen -- M model -- N normal -- MDA malondialdehyde -- MG muscle glycogen -- NF-κB nuclear factor kappa B -- NRF2 NF-E2-related factor 2 -- PCA principal component analysis -- PGC-1α peroxisome proliferator-activated receptor-γ coactivator-1 -- PI3K phosphoinositide 3-kinase -- SIRT1 sirtuin-1 -- SEM scanning electron microscope -- SOD superoxide dismutase -- T-AOC total antioxidant capacity -- TFAM mitochondrial transcription factor A -- TNF-α tumor necrosis factor-α -- VEGFA vascular endothelial growth factor A -- WP whey protein isolate -- XRD X-ray diffraction -- β-CD β-cyclodextrin; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105255
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Hawthorn polyphenol microcapsule (HPM) extract improves thermal processing stability. HPM reduces oxidative stress and inflammation in fatigued mice. HPM improves substrate and energy metabolism in fatigued mice. HPM alleviates fatigue in mice through AMPK pathway. HPM improves diversity of intestinal flora in fatigued mice. Abstract: This study prepared hawthorn polyphenol microcapsules (HPM) by microencapsulation and revealed that its potential mechanism of action in fatigued mice involves regulation of AMPK signaling pathway and balancing the intestinal microflora. Spray drying process improved thermal stability of hawthorn polyphenol. After HPM treatment, the weight-bearing swimming ability of fatigued mice was significantly improved (p < 0.05). HPM can effectively improve skeletal muscle substrate consumption and product metabolism and enhance antioxidant capacity (p < 0.05 or p < 0.01). Supplementation with HPM can provide energy through regulation of the PI3K/AKT/GSK-3Β pathway. HPM activated the AMPK pathway (p < 0.05 or p < 0.01) to improve mitochondrial dysfunction and cell metabolism and inhibited the NF-κB inflammatory pathway (p < 0.05) in fatigued mice. In addition, HPM can exhibit an anti-fatigue effect by improving the diversity of gut microbial species. Therefore, HPM can be used as a potential anti-fatigue nutritional supplement in functional foods to alleviate exercise fatigue.