A One-Health Sampling Strategy to Explore the Dissemination and Relationship Between Colistin Resistance in Human, Animal, and Environmental Sectors in Laos. (August 2022)

• Record Type: Journal Article
• Authors: Zhou, Yuqing; Farzana, Refath; Sihalath, Somsavanh; Rattanavong, Sayaphet; Vongsouvath, Manivanh; Mayxay, Mayfong; Sands, Kirsty; Newton, Paul N.; Dance, David A.B.; Hassan, Brekhna; Walsh, Timothy R.
• Is Part Of: Engineering. Volume 15(2022)
• Page Start: 45
• Page End: 56
• Publication Date: 2022-08
• Subjects: Colistin resistance -- mcr -- Escherichia coli -- Laos -- Horizontal transmission; Engineering -- Periodicals; Engineering -- China -- Periodicals; 620.005
• DOI: 10.1016/j.eng.2022.01.013
• Languages: English
• ISSNs: 2095-8099

Abstract

Abstract: This study was designed to investigate the molecular epidemiology of mobile colistin resistance ( mcr ) using a "One-Health" approach in Laos and to predict whether any dominant plasmid backbone and/or strain type influences the dissemination of mcr . We collected 673 samples from humans (rectal normal flora), poultry, and the environment (water, flies, birds, etc.) in Vientiane, Lao People's Democratic Republic (Laos), from May to September 2018. A total of 238 Escherichia coli ( E. coli ) isolated from non-duplicative samples, consisting of 98 MCR-positive E. coli (MCRPEC) (" mcr " denotes the gene encoding mobile colistin resistance, and "MCR" denotes the subsequent protein encoded by mcr ) and 140 MCR-negative E. coli (MCRNEC), were characterized by phenotype and Illumina sequencing. A subset of MCRPEC was selected for MinION sequencing, conjugation assay, plasmid stability, and growth kinetics in vitro . The prevalence of MCRPEC was found to be 14.6% (98/673), with the highest prevalence in human rectal swabs (45.9% (45/98), p < 0.0001, odds ratio (OR): 0.125, 95% confidence interval (CI): 0.077–0.202). The percentages of MCRPEC from other samples were 14.3% (2/14) in dog feces, 12.0% (24/200) in flies, 11.0% (11/100) in chicken meat, 8.9% (8/90) in chicken cloacal, 8.0% (4/50) in chicken caeca, and 7.5% (4/53) in wastewater. MCRPEC was significantly more resistant to co-amoxiclav, sulfamethoxazole-trimethoprim, levofloxacin, ciprofloxacin, and gentamicin than MCRNEC ( p < 0.05). Genomic analysis revealed the distribution of MCRPEC among diverse clonal types. The putative plasmid Inc types associated with mcr-1 were IncX4, IncHI2, IncP1, IncI2, and IncFIA, and those associated with mcr-3 were IncFII, IncFIA, IncFIB, IncP1, and IncR. Recovery of highly similar plasmids from both flies and other sampling sectors implied the role of flies in the dissemination of mcr-1 . mcr -positive plasmids were shown to be conjugative, and a significantly high transfer rate into a hypervirulent clone ST1193 was observed. Plasmids containing mcr irrespective of Inc type were highly stable and invariably did not exert a fitness effect upon introduction into a new host. These findings signify the urgent need for a standard infection control program to radically decontaminate the source of resistance.