Identification of a multiple drug-resistance gene island in the Haemophilus parasuis chromosome. (September 2020)

• Record Type: Journal Article
• Authors: Yang, Shoushen; Fan, Kewei; Lin, Weiming; Wang, Jiahui; Lin, Min; Yang, Song; Jiang, Yudan; Huang, Xiaoqian; Chen, Wenyan; Huang, Cuiqin
• Is Part Of: Journal of global antimicrobial resistance. Volume 22(2020)
• Page Start: 422
• Page End: 425
• Publication Date: 2020-09
• Subjects: Haemophilus parasuis -- ISApl1 -- blaROB-1 -- β-Lactamases -- Transmission; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2020.04.019
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Numerous drug-resistance genes including tet (B), bla ROB-1, sul2, catIII, aph(3″)-Ib, aph(6)-Id and aph(3′)-Ia were present in a Haemophilus parasuis chromosomal gene island as adjacent duplicate copies. The rep-orf3-bla ROB-1 structure most likely had a direct plasmid origin. The acquisition of bla ROB-1 and the other antibiotic resistance genes was related to the presence of IS Apl1 . Abstract: Objectives: The aim of this study was to determine the mechanisms and molecular characterisation of one strain (HPS412) of Haemophilus parasuis, which exhibited high MICs of antimicrobial susceptibility. Methods: A total of 113 H. parasuis strains isolated from pigs suffering from polyserositis, pneumonia or meningitis in China and screened them for antimicrobial susceptibility. Susceptibility testing of the minimal inhibitory concentrations (MIC) was determined in fastidious medium consisting of tryptone soya broth (TSB) containing 5% bovine serum and 10 μg/mL NAD in 96-well microtiter plates. The genomic DNA was completely sequenced by combining PacBio RS II and Illumina HiSeq 4000 platforms. Gene prediction was performed using Glimmer v.3.02 with Hidden Markov models. Results: One strain (HPS412) exhibited high MICs of sulfamethoxazole (256 μg/mL), tetracycline (128 μg/mL), streptomycin (128 μg/mL), gentamicin (128 μg/mL), amoxicillin (128 μg/mL), chloramphenicol (64 μg/mL), penicillin (64 μg/mL) and cefaclor (64 μg/mL). Sequence analysis showed that numerous drug-resistance genes including tet (B), bla ROB-1, sul2, catIII, aph(3″)-Ib, aph(6)-Id and aph(3′)-Ia were present in a chromosomal gene island as adjacent duplicate copies and the rep-orf3-bla ROB-1 structure most likely had a direct plasmid origin. The tet (B) and bla ROB-1 were flanked on one or both by IS Apl1 elements. Conclusions: The acquisition of bla ROB-1 and the other antibiotic resistance genes was related to the presence of IS Apl1 . IS Apl1 plays important roles in the horizontal transmission of antibiotic resistance genes.