Assessment of Cell Viability. Issue 1 (1st April 2013)

• Record Type: Journal Article
• Authors: Johnson, Simon; Nguyen, Vy; Coder, David
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Dobrucki, Jurek; Hyun, William C.; Nolan, John P.; Orfao, Alberto; Rabinovitch, Peter S.
• Is Part Of: Current protocols in cytometry. Volume 64:Issue 1(2013)
• Page Start: 9.2.1
• Page End: 9.2.26
• Publication Date: 2013-04-01
• Subjects: cytometry -- flow cytometry -- imaging -- probes -- toxicology -- assessment of cell toxicity; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/0471142956.cy0902s64
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: Cell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes or the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for determining the pre‐fixation viability of fixed cells either before or after fixation with amine‐reactive dyes suitable for a range of excitation wavelengths. Membrane‐impermeable dead cell and live cell dyes as well as dye‐exclusion procedures for microscopy are also included. Curr. Protoc. Cytom . 64:9.2.1‐9.2.26. © 2013 by John Wiley & Sons, Inc.