Cytometry in Malaria—A Practical Replacement for Microscopy?. Issue 1 (15th July 2013)

• Record Type: Journal Article
• Authors: Shapiro, Howard M.; Apte, Simon H.; Chojnowski, Grace M.; Hänscheid, Thomas; Rebelo, Maria; Grimberg, Brian T.
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Dobrucki, Jurek; Hyun, William C.; Nolan, John P.; Orfao, Alberto; Rabinovitch, Peter S.
• Is Part Of: Current protocols in cytometry. Volume 65:Issue 1(2013)
• Page Start: 11.20.1
• Page End: 11.20.23
• Publication Date: 2013-07-15
• Subjects: flow cytometry -- fluorescence -- hemozoin -- image cytometry -- malaria -- microscopy -- polarized light; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/0471142956.cy1120s65
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: Malaria, caused by protozoan Plasmodium parasites, kills ∼800, 000 people each year. Exact figures are uncertain because presumptive diagnoses are often made without identifying parasites in patients' blood either by microscopy, using Giemsa's century‐old stain, or by simpler tests that are ultimately dependent on microscopy for quality control. Microscopy itself relies on trained observers' ability to detect subtle morphological features of parasitized red blood cells, only a few of which may be present on a slide. Quantitative and objective flow cytometric measurements of cellular constituents such as DNA, RNA, and the malaria pigment hemozoin are now useful in research in malaria biology and pharmacology, and can provide more reliable identification of parasite species and developmental stages and better detection of low‐density parasitemia than could microscopy. The same measurements can now be implemented in much smaller, simpler, cheaper imaging cytometers, potentially providing a more accurate and precise diagnostic modality. Curr. Protoc. Cytom . 65:11.20.1‐11.20.23. © 2013 by John Wiley & Sons, Inc.