Contrast Enhancement in Light Microscopy. Issue 1 (1st January 2013)

• Record Type: Journal Article
• Authors: Ernst Keller, H.; Watkins, Simon
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Dobrucki, Jurek; Hyun, William C.; Nolan, John P.; Orfao, Alberto; Rabinovitch, Peter S.
• Is Part Of: Current protocols in cytometry. Volume 63:Issue 1(2013)
• Page Start: 2.1.1
• Page End: 2.1.9
• Publication Date: 2013-01-01
• Subjects: imaging and microscopy -- light microscopy -- instrumentation; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/0471142956.cy0201s63
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: The optical microscope is a fundamental component of an image cytometry system. This unit covers the basic concepts of light microscopy, including Köhler illumination, resolution, contrast, and numerical aperture, and reviews the many types of instruments and techniques for contrast enhancement. Curr. Protoc. Cytom . 63:2.1.1‐2.1.9. © 2013 by John Wiley & Sons, Inc.