Microbial characteristics of nitrifiers, denitrifiers and anammox bacteria on different support media to treat space mission wastewater. (15th February 2019)

• Record Type: Journal Article
• Authors: Dai, Fei; De Prá, Marina Celant; Vanotti, Matias B.; Gilmore, Kevin R.; Cumbie, William E.
• Is Part Of: Journal of environmental management. Volume 232(2019)
• Page Start: 943
• Page End: 951
• Publication Date: 2019-02-15
• Subjects: Fluorescence in-situ hybridization -- Biomass growth -- Early planetary base wastewater -- Biofilm support media; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2018.12.008
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: Biomass attachment and growth are important factors for the startup and stability of fixed-film biological reactors being proposed to recycle wastewater for potable water use in manned space activity. Eight different biofilm support media commonly used in wastewater treatment plants, aquaculture, and aquariums were compared for their relative ability to support attachment and growth of nitrifiers, denitrifiers, and anaerobic ammonia oxidizing (anammox) bacteria biomass. Accumulated total biomass was determined by comparing dry weight of each media before and after culturing of biomass. Fluorescence In-Situ Hybridization (FISH) analysis was used to quantify the proportion and relative activity of each organism group on each media. Measurements of dry biomass normalized to several media properties showed polyether polyurethane foam to have the highest extent of specific biomass attachment and colonization. Six of the eight media were able to sustain a population of anammox bacteria that was more abundant than the other cohorts. Graphical abstract: Image 1 Highlights: The best biofilm support media to treat space mission wastewater was determined. Growth of nitrifiers, denitrifiers and anammox on eight common media was compared. Fluorescence in-situ hybridization was utilized to quantify each organisms group. Dry biomass normalized to several media properties were evaluated for each media. Six of the eight media can sustain more anammox population than the other cohorts.