Basic Techniques in Mammalian Cell Tissue Culture. Issue 1 (1st November 2016)

• Record Type: Journal Article
• Authors: Phelan, Katy; May, Kristin M.
• Editors: Costa, Lucio G; Davila, Julio C.; Lawrence, David A.; Reed, Donald J.
• Is Part Of: Current protocols in toxicology. Volume 70:Issue 1(2016)
• Page Start: A.3B.1
• Page End: A.3B.22
• Publication Date: 2016-11-01
• Subjects: mammalian cells -- tissue culture -- aseptic technique -- medium -- passaging cells -- freezing cells; Toxicology -- Laboratory manuals; Toxicology -- methods; Toxicology; Laboratory Manual; Electronic books; Laboratory manuals; 571.95028
• DOI: 10.1002/cptx.13
• Languages: English
• ISSNs: 1934-9254

Abstract

Abstract: Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2016 by John Wiley & Sons, Inc.