Narrow Stimulated Resonance Raman Scattering and WGM Lasing in Small Conjugated Polymer Particles for Live Cell Tagging and Tracking. Issue 4 (9th December 2020)

• Record Type: Journal Article
• Authors: Haehnle, Bastian; Lamla, Markus; Sparrer, Konstantin M. J.; Gather, Malte C.; Kuehne, Alexander J. C.
• Is Part Of: Advanced optical materials. Volume 9:Issue 4(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-09
• Subjects: conjugated polymer particles -- laser emission -- live cell imaging -- whispering gallery modes; Optical materials -- Periodicals; Photonics -- Periodicals; 620.11295
• DOI: 10.1002/adom.202001553
• Languages: English
• ISSNs: 2195-1071

Abstract

Abstract: Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles is presented as a new method for generating narrow emission as an alternative to WGM‐based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. The synthesis of monodisperse micrometer‐sized poly(fluorene‐ co ‐divinylbenzene) particles is discussed and their WGM and SRRS emission are characterized. Finally, how these particles and their emission can be employed in live cell imaging and tagging is showcased. Abstract : Light‐emitting conjugated polymer particles with monodisperse diameters above 1 µm are developed. These particles act as whispering gallery mode (WGM) laser resonators for live cell imaging, tagging, and tracking. Particles that are too small to support WGM emission, instead, exhibit narrow laser‐like stimulated resonance Raman scattering (SRRS) emission, which is useful for multiplexed and multicolor live cell imaging.