PPARα Promotes Cancer Cell Glut1 Transcription Repression. Issue 6 (5th January 2017)

• Record Type: Journal Article
• Authors: You, Mengli; Jin, Jianhua; Liu, Qian; Xu, QingGang; Shi, Juanjuan; Hou, Yongzhong
• Is Part Of: Journal of cellular biochemistry. Volume 118:Issue 6(2017)
• Page Start: 1556
• Page End: 1562
• Publication Date: 2017-01-05
• Subjects: PPARα -- GLUT1 -- CELL SIGNALING -- TRANSCRIPTION REPRESSION; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.25817
• Languages: English
• ISSNs: 0730-2312

Abstract

ABSTRACT: Abundant nutrient availability including glucose and amino acids plays an important role in maintaining cancer cell energetic and biosynthetic pathways. As a nuclear receptor, peroxisome‐proliferator‐activated receptor α (PPARα) regulates inflammation and cancer progression, however, it is still unclear the interaction of PPARα with the cancer cell glucose metabolism. Here we found that PPARα reduced Glut1 (Glucose transporter 1) protein and gene levels in HCT‐116, SW480, HeLa, and MCF‐7 cancer cell lines. In contrast, silenced PPARα reversed this event. Further analysis shows that PPARα directly targeted the consensus PPRE motif of Glut1 promoter region resulting in Glut1 transcription repression. PPARα‐mediated Glut1 transcription repression led to decreased influx of glucose in cancer cells. These findings revealed a novel mechanism of PPARα‐mediated cancer cell Glut1 transcription repression. J. Cell. Biochem. 118: 1556–1562, 2017. © 2016 Wiley Periodicals, Inc. Abstract : As a nuclear receptor, peroxisome‐proliferator‐activated receptor α (PPARα) regulates inflammation and cancer progression, however, it is still unclear the interaction of PPARα with the cancer cell glucose metabolism. Here we found that PPARα directly inhibited Glut1 (Glucose transporter 1) transcription by targeting its promoter region leading to decreased influx of glucose. In contrast, silenced PPARα reversed this event. These findings revealed a novel mechanism of PPARα inhibited Glut1 transcription activity.