Platelet polyphosphate and energy metabolism in professional male athletes (soccer players): A cross‐sectional pilot study. Issue 15 (3rd August 2022)

• Record Type: Journal Article
• Authors: Ushiki, Takashi; Mochizuki, Tomoharu; Suzuki, Katsuya; Kamimura, Masami; Ishiguro, Hajime; Watanabe, Satoshi; Omori, Go; Yamamoto, Noriaki; Kawase, Tomoyuki
• Is Part Of: Physiological reports. Volume 10:Issue 15(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-08-03
• Subjects: athletes -- basal metabolic rate -- NADH -- platelets -- polyphosphates; Physiology -- Periodicals; 571
• DOI: 10.14814/phy2.15409
• Languages: English
• ISSNs: 2051-817X

Abstract

Abstract: Human platelet polyphosphate (polyP) is a multifunctional molecule; however, its functions are not yet fully understood. A recent study demonstrated that similar to skeletal muscle, polyP is involved in energy metabolism in platelets, which suggests that well‐trained athletes may exhibit elevated platelet polyP levels for energy storage. To test this hypothesis, we quantified platelet polyP along with NADH, a component involved in ATP production in non‐trained and well‐trained male Japanese participants of the same generation. Washed platelets were prepared from the venous blood of young, healthy, non‐athletes, and professional soccer players (pro‐athletes). NADH and polyP levels were spectrophotometrically determined using tetrazolium reduction and fluorometrically determined using 4′, 6‐diamidino‐2‐phenylindole at the excitation/emission wavelengths of 425/525 nm. Body weight and impedances were measured simultaneously. Statistical analyses were performed using the Mann–Whitney U test and Spearman correlation coefficient. Although basal metabolic rate levels were significantly higher, platelet polyP levels were significantly lower in pro‐athletes than in that in non‐athletes. No significant differences were detected in other body compositions or platelet indices between the two groups. The pro‐athlete group showed a moderate, nearly significant correlation ( R = 0.439; p = 0.0512) between platelet polyP and NADH levels. Taken together with the weak correlation data between polyP and body mass index, it is suggested that platelet polyP levels may be influenced by platelet and body energy metabolic activity. Further biochemical studies are needed to elucidate this mechanism. Abstract : Although polyphosphate (polyP) has recently been accepted as a metabolite of energy metabolism, its metabolism and function remain poorly explored in human platelets. It could be hypothesized that well‐trained professional athletes who generally exhibit higher basal metabolic rate than non‐athletes may also exhibit elevated levels of platelet polyP for energy storage. This cross‐sectional study highlights that against our hypothesis, platelet polyP levels are significantly lower in elite athletes than in non‐athletes.