A biuret‐derived, MS‐cleavable cross‐linking reagent for protein structural analysis: A proof‐of‐principle study. (9th December 2019)

• Record Type: Journal Article
• Authors: Hage, Christoph; Iacobucci, Claudio; Götze, Michael; Sinz, Andrea
• Is Part Of: Journal of mass spectrometry. Volume 55:Number 1(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-12-09
• Subjects: chemical cross‐linking -- cleavable cross‐linkers -- mass spectrometry -- peptides -- protein conformation; Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.4449
• Languages: English
• ISSNs: 1076-5174

Abstract

Abstract: Chemical cross‐linking combined with mass spectrometry (XL‐MS) and computational modeling has evolved as an alternative method to derive protein 3D structures and to map protein interaction networks. Special focus has been laid recently on the development and application of cross‐linkers that are cleavable by collisional activation as they yield distinct signatures in tandem mass spectra. Building on our experiences with cross‐linkers containing an MS‐labile urea group, we now present the biuret‐based, CID‐MS/MS‐cleavable cross‐linker imidodicarbonyl diimidazole (IDDI) and demonstrate its applicability for protein cross‐linking studies based on the four model peptides angiotensin II, MRFA, substance P, and thymopentin.