Front cover: Purification of anionic fluorescent probes through precise fraction collection with a two‐point detection system using multiple‐stacking preparative capillary transient isotachophoresis. Issue 13 (15th July 2020)

• Record Type: Journal Article
• Authors: Haraga, Tomoko; Tsujimura, Hiroto; Miyauchi, Saori; Kamimura, Takuya; Shibukawa, Masami; Saito, Shingo
• Is Part Of: Electrophoresis. Volume 41:Issue 13/14(2020)
• Page Start: NA
• Page End: NA
• Publication Date: 2020-07-15
• Subjects: Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.202070071
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract : DOI: 10.1002/elps.201900399 The cover picture shows the concept of the multiple stacking preparative capillary electrophoresis (MSP‐CE) for the purification of anionic fluorescent probes in aqueous solution. This new method was developed by combining an on‐capillary concentration‐separation technique based on capillary transient isotachophoresis of a large volume sample with multiple injections, and an accurate and precise fraction collection technique using a two‐point detection CE system. Fluorescent probe solutions of more than 99% purity were successfully obtained by MSP‐CE. This purification method is useful for the preparation of high‐purity anionic fluorescent reagent solutions available for ultratrace analysis in CE‐LIF.