Brain Sub/Region-Specific Effects of Olanzapine on c-Fos Expression of Chronically Socially Isolated Rats. (1st January 2019)

• Record Type: Journal Article
• Authors: Stanisavljević, Andrijana; Perić, Ivana; Gass, Peter; Inta, Dragos; Lang, Undine E.; Borgwardt, Stefan; Filipović, Dragana
• Is Part Of: Neuroscience. Volume 396(2019)
• Page Start: 46
• Page End: 65
• Publication Date: 2019-01-01
• Subjects: ABC avidin-biotinylated peroxidase -- AcbC accumbens nucleus, core -- AcbSh accumbens nucleus, shell -- ANOVA analysis of variance -- AP1 activator protein 1 family -- CA1d/v Cornu ammonis, area 1, dorsal/ventral -- CA2 Cornu ammonis area 2 -- CA3d/v Cornu ammonis area 3, dorsal/ventral -- Cg1 cingulate cortex -- CPu caudate putamen -- CSIS chronic social isolation -- DAB 3, 3′-diaminobenzidine tetrahydrochloride hydrate -- DGd/v dentate gyrus dorsal/ventral -- DMH dorsomedial hypothalamic nucleus -- dHIPP dorsal hippocampus -- DP dorsopeduncular cortex -- EPSEs extrapyramidal side effects -- HTH hypothalamus -- IL infralimbic cortex -- i.p. intraperitoneal -- MD major depression -- MB marble burying -- mPFC medial prefrontal cortex -- NGS normal goat serum -- NAc nucleus accumbens -- Olz olanzapine -- PBS phosphate-buffered saline -- PCP-4 Purkinje cell protein marker 4 -- PrL prelimbic cortex -- PVP paraventricular thalamic nucleus, posterior part -- RSC retrosplenial cortex -- RSD retrosplenial dysgranular cortex -- RSGc retrosplenial granular cortex, c region -- RT room temperature -- SP sucrose preference Veh, vehicle -- vHIPP ventral hippocampus -- VMH ventromedial hypothalamic nucleus; depression- and anxiety-like behaviors -- social isolation -- C-fos -- olanzapine -- brain sub/regions; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2018.11.015
• Languages: English
• ISSNs: 0306-4522

Abstract

Graphical abstract: Highlights: Olz reverses depression- and anxiety-like behaviors induced by CSIS. CSIS increases c-Fos protein expression in stress-relevant brain sub/regions. Olz shows region-specific effects on c-Fos protein expression. Olz in controls shows most pronounced changes if c-Fos protein expression in CPu. Abstract: Olanzapine (Olz) is an atypical antipsychotic used to treat depression, anxiety and schizophrenia, which can be caused by chronic psychosocial stress. c-Fos protein expression has been used as an indirect marker of neuronal activity in response to various forms of stress or pharmacological treatments. We examined the effects of a 3-week treatment of Olz (7.5 mg/kg/day) on c-Fos protein expression in stress-relevant brain sub/regions, its relationship with isolation-induced behavioral changes, and potential sites of Olz action on control and male rats exposed to 6 weeks of chronic social isolation (CSIS), an animal model of depression. Olz treatment reversed depression- and anxiety-like behaviors induced by CSIS and suppressed a CSIS-induced increase in the number of c-Fos-positive cells in subregions of the dorsal hippocampus, ventral (v) DG, retrosplenial cortex, and medial prefrontal cortex. In contrast, no change in c-Fos expression was seen in the CA3v, amygdala and thalamic, hypothalamic or striatal subregions in Olz-treated CSIS rats, suggesting different brain sub/regions' susceptibility to Olz. An increased number of c-Fos-positive cells in the CA1v, amygdala and thalamic, hypothalamic and striatal subregions in controls as well as in the CA1v and subregion of the hypothalamus and nucleus accumbens in Olz-treated CSIS rats was found. Results suggest the activation of brain sub/regions following CSIS that may be involved in depressive and anxiety-like behaviors. Olz treatment showed region-specific effects on neuronal activation. Our data contribute to a better understanding of the mechanisms underlying the CSIS response and potential brain targets of Olz in socially isolated rats.