Microbacterium algeriense sp. nov., a novel actinobacterium isolated from Algerian oil production waters. Issue 12 (2nd December 2020)

• Record Type: Journal Article
• Authors: Lenchi, Nesrine; Anzil, Adriana; Servais, Pierre; Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Llirós, Marc
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 12(2020)
• Page Start: 6044
• Page End: 6051
• Publication Date: 2020-12-02
• Subjects: actinobacteria -- Microbacterium algeriense sp. nov. -- oilfield -- production water; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004434
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A non-motile, straight-rod-shaped, Gram-stain-positive and facultative anaerobic bacterium (i . e., strain G1 T ) was isolated from production waters from an Algerian oilfield. Growth was observed in the presence of 0.3–3.5 % (w/v) NaCl, at 20–50 °C and at pH 6.0–9.0. Results of phylogenetic analyses based on 16S rRNA gene sequences showed that strain G1 T belonged to the genus Microbacterium . Strain G1 T was closely related to Microbacterium oxydans (DSM 20578 T ) and Microbacterium maritypicum (DSM 12512 T ) with 99.8 % sequence similarity and to Microbacterium saperdae (DSM 20169 T ) with 99.6 % sequence similarity. Strain G1 T contained MK9, MK10, MK11, MK12 and MK13 as respiratory quinones, and phosphatidylglycerol, diphosphatidylglycerol and glycolipid as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0 . The estimated DNA G+C content was 69.57 mol% based on its draft genome sequence. Genome annotation of strain G1 T predicted the presence of 3511 genes, of which 3483 were protein-coding and 47 were tRNA genes. The DNA–DNA hybridization (DDH) and average nucleotide identity (ANI) values between strain G1 T and M. oxydans (DSM 20578 T ) and M. maritypicum (DSM 12512 T ) were in both cases far below the respective species boundary thresholds (27.5 and 28.0 % for DDH; and 84.40 and 84.82% for ANI, respectively). Based on the data presented above, strain G1 T was considered to represent a novel species for which the name Microbacterium algeriense is proposed with the type strain G1 T (=DSM 109018 T =LMG 31276 T ).